Development of an Immunochromatographic Test Strip for Rapid Simultaneous Detection of Enrofloxacin and Ofloxacin in Tissue of Chicken Muscle and Pork

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• 作者：Yuchen Wu ; Shuang Guo ; Qing Dong ; Yuzhen Song
• 关键词：Monoclonal antibody ; Enrofloxacin and ofloxacin ; Immunochromatographic strip ; Simultaneous detection
• 刊名：Food Analytical Methods
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：9
• 期：10
• 页码：2807-2813
• 全文大小：473 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Food Science
  Chemistry
  Microbiology
  Analytical Chemistry
  
• 出版者：Springer New York
• ISSN：1936-976X
• 卷排序：9

文摘

ENR and OFL are the most consumed quinolones on livestock in China. In this work, we developed a rapid immunochromatographic lateral flow test strip for simultaneous detection of the residues of enrofloxacin and ofloxacin in chicken muscle and pork. We screened an anti-ENR and OFL monoclonal antibody. The IC₅₀ of anti-ENR and anti-OFL were 6.67 and 7.13 ng/ml, respectively. The present immunochromatographic lateral flow test strip is a one-step assay and required much less professional personnel and experimental instruments. In the present study, the decision limit (CCα) of the test strip was calculated to be 0.089 ng/mL and detection capability (CCβ) was 0.217 ng/mL with the scanner. The limit of detection was estimated to be 10 ng/mL. According to parallel HPLC analysis with 47 blind samples, coincidence rate is 100 % when the contents of ENR and OFL were more than 10 ng/mL. Results indicated that the strip test we developed had good reliability, and the strip test gave neither false positive nor false negative results. It will provide results within 20 min without special equipment. Therefore, the test strip is very useful as a screening method for semi-quantitative or qualitative detection of enrofloxacin and ofloxacin in chicken muscle and pork.