Detection of morphologic alterations in rectal carcinoma following preoperative radiochemotherapy based on multiphoton microscopy imaging

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• 作者：Lianhuang Li (1)
  Zhifen Chen (2)
  Xingfu Wang (3)
  Hongsheng Li (1)
  Weizhong Jiang (2)
  Shuangmu Zhuo (1)
  Guoxian Guan (2)
  Jianxin Chen (1)
  
  1. Institute of Laser and Optoelectronics Technology ; Fujian Provincial Key Laboratory for Photonics Technology ; Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education ; Fujian Normal University ; Fuzhou ; 350007 ; China
  2. Department of Colorectal Surgery ; The Affiliated Union Hospital ; Fujian Medical University ; Fuzhou ; 350001 ; China
  3. Department of Pathology ; The First Affiliated Hospital ; Fujian Medical University ; Fuzhou ; 350001 ; China
  
• 关键词：Multiphoton microscopy ; Preoperative radiochemotherapy ; Fibrosis ; Two ; photon excited fluorescence ; Second harmonic generation
• 刊名：BMC Cancer
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：15
• 期：1
• 全文大小：1,743 KB
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• 刊物主题：Cancer Research; Oncology; Stem Cells; Animal Models; Internal Medicine;
• 出版者：BioMed Central
• ISSN：1471-2407

文摘

Background Preoperative radiochemotherapy improves outcomes in patients with locally advanced rectal carcinoma, and has been used increasingly in patient management. However, there is a strong clinical need to assess tumor response to neoadjuvant treatment, and a non-invasive technique that allows the precise identification of morphologic changes in tumors would be of considerable clinical interest. Methods In this study, we used multiphoton microscopy (MPM) to detect morphologic alterations in rectal adenocarcinomas in patients treated with preoperative radiochemotherapy. Results MPM was able to identify histopathologic alterations in rectal cancer following preoperative radiochemotherapy, and allowed the qualitative assessment of treatment efficacy and feasibility in relation to dose or strategy. Conclusion These findings may provide the groundwork for evaluating tumor response to neoadjuvant treatment, thus allowing the tailoring of effective treatment doses and strategies.