MicroRNA124 Regulated Neurite Elongation by Targeting OSBP

详细信息    查看全文

• 作者：Xi Gu ; Aili Li ; Shuhu Liu ; Lifang Lin ; Shuang Xu ; Peidong Zhang…
• 关键词：miR ; 124 ; OSBP ; Neurite elongation ; Brain development
• 刊名：Molecular Neurobiology
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：53
• 期：9
• 页码：6388-6396
• 全文大小：1,924 KB
• 刊物主题：Neurosciences; Neurobiology; Cell Biology; Neurology;
• 出版者：Springer US
• ISSN：1559-1182
• 卷排序：53

文摘

MicroRNA-124 (miR-124), a brain-specific miRNA molecule, has been implicated in stimulating neurite outgrowth and elongation during neuronal differentiation. However, the direct target genes and the mechanisms of miR-124-induced neurite outgrowth are poorly understood. In this study, we demonstrated that miR-124 directly targeted and downregulated the endogenous expression of oxysterol-binding protein (OSBP). A previous study found that the expression of miR-124 increased during brain development. In the present study, we demonstrated that the expression of OSBP decreased during the development of the C57BL/6 mouse cortex, which was negatively correlated with miR-124 expression. OSBP knockdown using specific shRNAs promoted neurite outgrowth and elongation in both Neuro-2a cells and primary cultured mouse cortical neurons. Conversely, OSBP overexpression strongly repressed the neurite elongation-enhancing effect of miR-124 in Neuro-2a cells. Our results suggested that OSBP may be a target and downstream effector of miR-124 for regulating neurite outgrowth and elongation.