Evaluation of salt tolerance of transgenic tobacco plants bearing the P5CS1 gene of Arabidopsis thaliana

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• 作者：S. M. Ibragimova ; E. A. Trifonova ; E. A. Filipenko…
• 关键词：genetic engineering ; proline metabolism genes ; resistance ; stress
• 刊名：Russian Journal of Genetics
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：51
• 期：12
• 页码：1181-1188
• 全文大小：1,866 KB
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• 作者单位：S. M. Ibragimova (1)
  E. A. Trifonova (1)
  E. A. Filipenko (1)
  V. K. Shymny (1) (2)
  
  1. Institute of Cytology and Genetics Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia
  2. Department of Cytology and Genetics, Novosibirsk State University, Novosibirsk, 630090, Russia
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Human Genetics
  Animal Genetics and Genomics
  Microbial Genetics and Genomics
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-3369

文摘

Arabidopsis thaliana Δ1-pyrroline-5-carhoxylate synthetase 1 (P5CS1) cDNA was cloned under the control of the potent constitutive 35S RNA promoter of the cauliflower mosaic virus and transferred into genome of tobacco cv. Petit Havana SR-1 (Nicotiana tabacum L.) plants. It is shown that the constitutive level of proline in the transgenic plants T₀ exceeds that of the SR1 reference line by 1.5 to 4 times. Under conditions of salt stress (200, 300 mM NaCl) T₁-generation transgenic plants in early stages of development formed a large biomass, developed more quickly, and had a higher rate of root growth compared to the control, which confirms the involvement of the P5CS1 gene in molecular mechanisms of stress resistance in plants. Keywords genetic engineering proline metabolism genes resistance stress