A panel of genic single nucleotide polymorphism (SNP) markers for the Pacific abalone, Haliotis discus hannai
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- 作者：Zhen Zhang (1) (2)
Aibin Zhan (3)
Xiao Liu (1) - 关键词：Pacific abalone ; Haliotis discus hannai Ino ; SNP ; PAMSA
- 刊名：Conservation Genetics Resources
- 出版年：2010
- 出版时间：September 2010
- 年：2010
- 卷：2
- 期：1-supp
- 页码：133-135
- 全文大小：162KB
- 参考文献：1. Gaudet M, Fara A-G, Sabatti M (2007) Single-reaction for SNP genotyping on agarose gel by allele-specific PCR in black poplar ( / Populus nigra / L.). Plant Mol Biol Report 25:1-
2. Qi HG, Liu X, Zhang GF (2008) Characterization of 12 single nucleotide polymorphisms (SNPs) in Pacific abalone, / Haliotis discus hannai. Mol Ecol Resour 8:974-76 CrossRef
3. Qi HG, Liu X, Zhang GF, Wu FC (2009) Mining expressed sequences for single nucleotide polymorphisms in Pacific abalone / Haliotis discus hannai. Aquac Res 40:1661-667 CrossRef
4. Sambrook J, Fritsch E, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York - 作者单位：Zhen Zhang (1) (2)
Aibin Zhan (3)
Xiao Liu (1)
1. Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, China
2. Graduate School, Chinese Academy of Sciences, 100039, Beijing, China
3. Great Lakes Institute for Environmental Research, University of Windsor, 401 Sunset Avenue, Windsor, ON, N9B 3P4, Canada
文摘
We developed 14 single nucleotide polymorphism (SNP) markers for the Pacific abalone, Haliotis discus hannai, one valuable fishery resource in East Asian countries and popular seafood worldwide. The polymorphisms of these SNP loci were assessed using a wild population comprising 37 unrelated individuals. The minor allele frequency ranged from 0.1216 to 0.4865, and the observed and expected heterozygosities ranged from 0.189 to 0.865 and from 0.217 to 0.507, respectively. Only one locus (HdS82) deviated significantly from Hardy–Weinberg equilibrium and no significant linkage disequilibrium was detected after Bonferroni corrections. These polymorphic markers appear suitable for conservation surveys and population genetic studies for the Pacific abalone.