Laser-Induced Acoustic Desorption Atmospheric Pressure Photoionization via VUV-Generating Microplasmas
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- 作者：Kevin Benham ; Robert Hodyss…
- 关键词：Laser ; induced acoustic desorption ; Atmospheric pressure photoionization ; Microplasma ; Microhollow cathode discharge ; Low ; polarity analytes
- 刊名：Journal of The American Society for Mass Spectrometry
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：27
- 期：11
- 页码：1805-1812
- 全文大小：1,403 KB
- 刊物主题：Analytical Chemistry; Biotechnology; Organic Chemistry; Proteomics; Bioinformatics;
- 出版者：Springer US
- ISSN：1879-1123
- 卷排序：27
文摘
We demonstrate the first application of laser-induced acoustic desorption (LIAD) and atmospheric pressure photoionization (APPI) as a mass spectrometric method for detecting low-polarity organics. This was accomplished using a Lyman-α (10.2 eV) photon generating microhollow cathode discharge (MHCD) microplasma photon source in conjunction with the addition of a gas-phase molecular dopant. This combination provided a soft desorption and a relatively soft ionization technique. Selected compounds analyzed include α-tocopherol, perylene, cholesterol, phenanthrene, phylloquinone, and squalene. Detectable surface concentrations as low as a few pmol per spot sampled were achievable using test molecules. The combination of LIAD and APPI provided a soft desorption and ionization technique that can allow detection of labile, low-polarity, structurally complex molecules over a wide mass range with minimal fragmentation.