Elicitation of galanthamine and lycorine biosynthesis by Leucojum aestivum L. and L. aestivum ‘Gravety Giant’ plants cultured in bioreactor RITA®
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- 作者：Agata Ptak ; Emilia Morańska ; Sahar Saliba…
- 关键词：Amaryllidaceae alkaloids ; 1 ; Aminocyclopropane ; 1 ; carboxylic acid ; Ethephon ; Methyl jasmonate ; Salicylic acid ; In vitro plant
- 刊名：Plant Cell, Tissue and Organ Culture (PCTOC)
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：128
- 期：2
- 页码：335-345
- 全文大小：
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Plant Sciences; Plant Physiology; Plant Genetics & Genomics; Plant Pathology;
- 出版者：Springer Netherlands
- ISSN：1573-5044
- 卷排序：128
文摘
In order to establish an attractive method for the production of valuable medicinal alkaloids (galanthamine and lycorine), the plants of Leucojum aestivum and L. aestivum ‘Gravety Giant’ grown in bioreactor RITA® were subjected to various concentrations of methyl jasmonate (MeJA), salicylic acid (SA), 1-aminocyclopropane-1-carboxylic acid (ACC) and 2-chloroethylphosphonic acid (ethephon) at different times of culture. The application of MeJA showed a negative effect on L. aestivum and L. aestivum ‘Gravety Giant’ plant growth. We observed that the incubation of plants during 168 h with 100 µM of MeJA resulted above two times lower F.W. (fresh weight) increments compared with control. While SA showed an inhibitory effect only on the growth of L. aestivum cultures. ACC and ethephon had a positive effect on both types of culture. Treatment with 50 µM of MeJA during 168 h stimulated galanthamine and lycorine biosynthesis in L. aestivum and L. aestivum ‘Gravety Giant’ cultures. In addition, the accumulation of galanthamine was increased when 10 µM of ACC were added to both types of culture. 10 µM of ACC stimulated also lycorine biosynthesis by L. aestivum ‘Gravety Giant’. The addition of 10 µM of ethephon had a positive effect only on lycorine production in plants of L. aestivum. SA promoted galanthamine and lycorine biosynthesis in tested plants. Indeed the highest galanthamine (0.8 mg/g dry weight: D.W.) and lycorine (1.53 mg/g D.W.) concentrations were observed in L. aestivum ‘Gravety Giant’ plants treated with 5 µM of SA during 10 h.