Plethysmography measurements of respiratory function in conscious unrestrained mice

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• 作者：John C. Quindry ; Christopher G. Ballmann…
• 关键词：Mdx ; Respiration ; Whole body plethysmography
• 刊名：The Journal of Physiological Sciences
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：66
• 期：2
• 页码：157-164
• 全文大小：435 KB
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• 作者单位：John C. Quindry (1)
  Christopher G. Ballmann (1)
  Erin E. Epstein (1)
  Joshua T. Selsby (2)
  
  1. Cardioprotection Laboratory, School of Kinesiology, Auburn University, Auburn, AL, 36830, USA
  2. Animal Sciences, Iowa State University, Ames, IA, USA
  
• 刊物主题：Human Physiology; Neurosciences; Animal Biochemistry; Animal Physiology; Cell Physiology; Neurobiology;
• 出版者：Springer Japan
• ISSN：1880-6562

文摘

Whole body plethysmography (WBP) is used to quantify pulmonary function in conscious, unrestrained mice. We determined currently whether time of day and environmental lighting influence day-to-day reproducibility of pulmonary function, and quantifed the necessary habituation time in the WBP chamber. Two-month-old male C57BL6 and mdx mice (n = 8/group, reverse light cycle), were examined on consecutive days using a calibrated WBP chamber and manufacturer software was used to calculate respiratory measures. Respiratory data stabilized between 5–10 min for all variables. Mice exhibited time of day respiratory differences, performing more forceful and less frequent breaths midday (11:45 a.m. and 3:00  p.m.) compared to 7:30 a.m. WBP performed in darkened conditions elicited more forceful breathing than lit conditions. Day-to-day reproducibility during controlled conditions ranged from r 2 = 0.58 to 0.62 for the functional measures. Findings indicate reproducible respiratory data are obtainable following a 15-min chamber habituation and standardization of time of day and room lighting. Keywords Mdx Respiration Whole body plethysmography