Different distribution patterns of ten virulence genes in Legionella reference strains and strains isolated from environmental water and patients

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• 作者：Xiao-Yong Zhan ; Chao-Hui Hu ; Qing-Yi Zhu
• 关键词：Legionella ; Virulence genes ; Distribution pattern ; Virulence evolution
• 刊名：Archives of Microbiology
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：198
• 期：3
• 页码：241-250
• 全文大小：1,100 KB
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• 作者单位：Xiao-Yong Zhan (1) (2)
  Chao-Hui Hu (1) (2)
  Qing-Yi Zhu (1) (2)
  
  1. Guangzhou KingMed Center for Clinical Laboratory, No.10, Luoxuan 3 Road, Guangzhou International Bio-island, Guangzhou, 510300, China
  2. KingMed School of Laboratory Medicine, Guangzhou Medical University, Guangzhou, 510330, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Microbial Ecology
  Biochemistry
  Cell Biology
  Biotechnology
  Ecology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-072X

文摘

Virulence genes are distinct regions of DNA which are present in the genome of pathogenic bacteria and absent in nonpathogenic strains of the same or related species. Virulence genes are frequently associated with bacterial pathogenicity in genus Legionella. In the present study, an assay was performed to detect ten virulence genes, including iraA, iraB, lvrA, lvrB, lvhD, cpxR, cpxA, dotA, icmC and icmD in different pathogenicity islands of 47 Legionella reference strains, 235 environmental strains isolated from water, and 4 clinical strains isolated from the lung tissue of pneumonia patients. The distribution frequencies of these genes in reference or/and environmental L. pneumophila strains were much higher than those in reference non-L. pneumophila or/and environmental non-L. pneumophila strains, respectively. L. pneumophila clinical strains also maintained higher frequencies of these genes compared to four other types of Legionella strains. Distribution frequencies of these genes in reference L. pneumophila strains were similar to those in environmental L. pneumophila strains. In contrast, environmental non-L. pneumophila maintained higher frequencies of these genes compared to those found in reference non-L. pneumophila strains. This study illustrates the association of virulence genes with Legionella pathogenicity and reveals the possible virulence evolution of non-L. pneumophia strains isolated from environmental water.