PNKP knockdown by RNA interference inhibits herpes simplex virus-1 replication in astrocytes

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• 作者：Lei Yue (1)
  Sujie Guo (1)
  Xia Cao (2)
  Ying Zhang (1)
  Le Sun (1)
  Longding Liu (1)
  Min Yan (1)
  Qihan Li (1)
  
• 关键词：Herpes simplex virus I (HSV ; 1) ; Replication ; PNKP ; Cyclization ; Primary culture ; Monkey
• 刊名：Virologica Sinica
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：28
• 期：6
• 页码：345-351
• 全文大小：
• 作者单位：Lei Yue (1)
  Sujie Guo (1)
  Xia Cao (2)
  Ying Zhang (1)
  Le Sun (1)
  Longding Liu (1)
  Min Yan (1)
  Qihan Li (1)
  
  1. Institute of Medical Biology, Chinese Academy of Medicine Science & Peking Union Medical College, Kunming, 650118, China
  2. The Second Affiliated Hospital of Kunming Medical University, Kunming, 650101, China
  
• ISSN：1995-820X

文摘

Herpes simplex virus-1 (HSV-1) is a major pathogen that causes various central nervous system (CNS) diseases, including herpes simplex encephalitis and meningitis. According to recent studies, PNKP significantly affects the proliferation of HSV-1 in astrocytes. Here, we used viral proliferation curves to confirm the significant inhibitory effects of PNKP on HSV-1 proliferation. PNKP downregulation was also confirmed by analyzing the transcription of viral genes. We found that PNKP downregulation affects the viral DNA copy number. This study preliminarily confirms that PNKP affects viral proliferation by affecting HSV-1 genome cyclization. These results also suggest that astrocytes play a specific role in preventing HSV-1 infection.