Square-edge intraocular lenses and epithelial lens cell proliferation: implications on posterior capsule opacification in an in vitro model
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- 作者：Rita Mencucci (1)
Eleonora Favuzza (1)
Carlotta Boccalini (1)
Jean-Jacques Gicquel (2)
Laura Raimondi (3)
1. Department of Surgery and Translational Medicine -Eye Clinic ; University of Florence ; Florence ; Italy
2. Department of Ophthalmology ; Poitiers University Hospital ; Poitiers ; Cedex ; France
3. Department of NEUROFARBA ; section of Pharmacology ; University of Florence ; Florence ; Italy - 关键词：Posterior capsule opacification ; Intraocular lens ; Square edge ; Cataract
- 刊名：BMC Ophthalmology
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：15
- 期：1
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10. Dawes LJ, Illingworth CD, Wormstone I: A fully human in vitro capsular bag model to permit intraocular lens evaluation. / Invest Ophthalmol Vis Sci 2012, 53:23-. 10.1167/iovs.11-8851 CrossRef
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12. Burger J, Kreutzer T, Alge CS, Strauss RW, Eibl K, Haritoglou C, / et al.: Capsular tension - 刊物主题：Ophthalmology;
- 出版者：BioMed Central
- ISSN：1471-2415
文摘
Background To evaluate lens epithelial cell (LEC) proliferation with two different designs (one-piece or three-piece) of hydrophobic acrylic IOLs with 360° square optic edge using an in vitro culture model of posterior capsule opacification (PCO). Methods This experimental study was conducted at the Department of NEUROFARBA, Section of Pharmacology, University of Florence, Italy. Human LECs were seeded and cultured in transwell cell culture inserts coated with a type-IV collagen membrane on which an IOL (one-piece Tecnis-1 or three-piece AR40E, Abbott Medical Optics Inc.) had been previously placed. As control, cells were plated on the insert membrane without an IOL. At day six (cells confluent in controls) IOLs were removed and cell counting, viability and cell density under and outside the IOLs were evaluated. Results No statistically significant difference in the number of cells (p--.05) between inserts with the one-piece and three-piece IOLs was found. Cell density in the area under each IOL was significantly lower than in the area outside of it (p--.05), or in the control insert. (p--.05). Cell density under the single-piece IOL was not significantly different from that under the three-piece IOL (p--.05). Conclusions A 360° sharp-edge played a crucial role in avoiding LEC migration under the IOL and preventing the formation of PCO after cataract surgery. Long term clinical evaluation is necessary to estimate functional results.