Surface antigenic profiling of stem cells from human omentum fat in comparison with subcutaneous fat and bone marrow

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• 作者：M. Dhanasekaran (1) dhanasekarbio@gmail.com
  S. Indumathi (2)
  A. Kanmani (1)
  R. Poojitha (1)
  K. M. Revathy (1)
  J. S. Rajkumar (1)
  D. Sudarsanam (2)
• 关键词：Omentum fat derived stem cells – ; Cell surface marker – ; Mesenchymal stem cells – ; Cell adhesion molecules – ; Flowcytometry
• 刊名：Cytotechnology
• 出版年：2012
• 出版时间：October 2012
• 年：2012
• 卷：64
• 期：5
• 页码：497-509
• 全文大小：1.0 MB
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• 作者单位：1. Lifeline Multispeciality Hospital, Perungudi, Chennai, India2. Loyola College, Nungambakkam, Chennai, India
• ISSN：1573-0778

文摘

Omentum fat derived stem cells have emerged as an alternative and accessible therapeutic tool in recent years in contrast to the existing persuasive sources of stem cells, bone marrow and subcutaneous adipose tissue. However, there has been a scanty citation on human omentum fat derived stem cells. Furthermore, identification of specific cell surface markers among aforesaid sources is still controversial. In lieu of this existing perplexity, the current research work aims at signifying omentum fat as a ground-breaking source of stem cells by surface antigenic profiling of stem cell population. In this study, we examined and compared the profiling of cell surface antigenic expressions of hematopoietic stem cells, mesenchymal stem cells, cell adhesion molecules and other unique markers such as ABCG2, ALDH and CD 117 in whole cell population of human omentum fat, subcutaneous fat and bone marrow. The phenotypic characterization through flowcytometry revealed the positive expressions of CD 34, CD 45, CD 133, HLADR, CD 90, CD 105, CD 73, CD 29, CD 13, CD 44, CD 54, CD 31, ALDH and CD 117 in all sources. The similarities between the phenotypic expressions of omentum fat derived stem cells to that of subcutaneous fat and bone marrow substantiates that identification of ultimate source for curative therapeutics is arduous to assess. Nevertheless, these results support the potential therapeutic application of omentum fat derived stem cells.