Molecular identification of Sarcocystis rileyi sporocysts in red foxes (Vulpes vulpes) and raccoon dogs (Nyctereutes procyonoides) in Lithuania

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• 作者：Petras Prakas ; Simona Liaugaudait? ; Liuda Kutkien? ; Aniolas Sruoga…
• 关键词：Sarcocystis rileyi ; Oocysts/sporocysts ; Vulpes vulpes ; Nyctereutes procyonoides ; ITS ; 1 ; Species ; specific PCR
• 刊名：Parasitology Research
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：114
• 期：5
• 页码：1671-1676
• 全文大小：559 KB
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• 作者单位：Petras Prakas (1) (2)
  Simona Liaugaudait? (1)
  Liuda Kutkien? (1)
  Aniolas Sruoga (1) (3)
  Saulius ?va?as (1)
  
  1. Institute of Ecology, Nature Research Centre, Akademijos 2, 08412, Vilnius, Lithuania
  2. Institute of Biology Systems and Genetic Research, Veterinary Academy, Lithuanian University of Health Sciences, Til??s 18, 47181, Kaunas, Lithuania
  3. Vytautas Magnus University, K. Donelai?io 58, 44248, Kaunas, Lithuania
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Medical Microbiology
  Microbiology
  Immunology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-1955

文摘

Despite the fact that Sarcocystis rileyi is one of the earliest described species of the genus Sarcocystis forming macrocysts in ducks, the life cycle of this species is still unknown in Europe. Sarcocystis spp. oocysts/sporocysts were observed in faeces of four of 23 (17.4?%) and in small intestine mucosal scrapings of four of 20 (20.0?%) red foxes (Vulpes vulpes) and in small intestine mucosal scrapings of seven of 13 (53.8?%) raccoon dogs (Nyctereutes procyonoides) hunted in Lithuania. A very small number of Sarcocystis sporocysts measuring 11.9?×-.3?μm (n--) was found in faecal samples, whereas considerably more sporulated Sarcocystis oocysts and free sporocysts were detected in the small intestines of red foxes and raccoon dogs. These sporocysts measured 12.9?×-.1?μm (n--6) and 12.1?×-.1?μm (n--4) in red foxes and raccoon dogs, respectively. Using species-specific PCR and subsequent sequencing, internal transcribed spacer 1 (ITS-1) region partial sequences of oocysts/sporocysts from small intestine mucosal scrapings of six raccoon dogs and three red foxes were identified as belonging to S. rileyi. The present study provides strong evidence showing that the red fox and the raccoon dog can serve as final hosts of S. rileyi in Europe; however, transmission experiments are needed for the ultimate approval.