TdCBL6, a calcineurin B-like gene from wild emmer wheat (Triticum dicoccoides), is involved in response to salt and low-K+ stresses
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- 作者：Liang Chen (1)
Jing Ren (1) (2)
Haiyan Shi (3)
Yongkuan Zhang (1)
Yu You (4)
Jibiao Fan (1) (2)
Ke Chen (1)
Shuqian Liu (1) (2)
Eviatar Nevo (5)
Jinming Fu (1)
Junhua Peng (1) (6) - 关键词：Wild emmer wheat ; CBL protein ; Abiotic stress ; Salt tolerance ; Low ; K+
- 刊名：Molecular Breeding
- 出版年：2015
- 出版时间：January 2015
- 年：2015
- 卷：35
- 期：1
- 全文大小：1,701 KB
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Jing Ren (1) (2)
Haiyan Shi (3)
Yongkuan Zhang (1)
Yu You (4)
Jibiao Fan (1) (2)
Ke Chen (1)
Shuqian Liu (1) (2)
Eviatar Nevo (5)
Jinming Fu (1)
Junhua Peng (1) (6)
1. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Institute/Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, Hubei, China
2. Graduate University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049, China
3. College of Horticulture, Agricultural University of Hebei, Baoding, 071001, China
4. Huiyang Sun Yat-Sen Middle School, Huizhou, 516211, Guangdong, China
5. Institute of Evolution, University of Haifa, 31905, Mount Carmel, Haifa, Israel
6. Department of Soil and Crop Sciences, Colorado State University, Fort Collins, CO, 80523, USA - 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Plant Sciences - 出版者：Springer Netherlands
- ISSN：1572-9788
文摘
The calcineurin B-like proteins (CBLs), a unique family of calcium sensors in plants, have been shown to be involved in abiotic stresses, such as salt, drought and cold. Although extensive studies and remarkable progress have been made in Arabidopsis (Arabidopsis thaliana) CBLs, very little is known about the role of CBL genes in wheat. In this study, a CBL gene, designated TdCBL6, was cloned and characterized from wild emmer wheat (Triticum dicoccoides), the progenitor of cultivated wheat. Sequence alignment revealed that TdCBL6 shares high sequence homology with rice OsCBL6. Phylogenetic analysis also revealed that TdCBL6 protein has the closest evolutionary relationship with rice OsCBL6 protein. TdCBL6 transcription was induced by NaCl, polyethylene glycol and abscisic acid. Further differential expression analysis revealed that TdCBL6 expression was much higher in the salt-tolerant line than in the salt-sensitive line when they were subjected to salt treatment. Transgenic Arabidopsis ectopic expression of the TdCBL6 gene displayed higher levels of photosynthetic efficiency (Fv/Fm) and lower ion leakage (EL) than wild-type (WT) plants under NaCl stress conditions. Moreover, TdCBL6-overexpressing lines showed low-K+ (LK)-sensitive phenotypes compared with WT plants. Further experiments revealed that ectopic expression of TdCBL6 resulted in reduction of H2O2 content, and affected expression of K+-responsive/H2O2-regulated genes under LK stress. Taken together, we demonstrated that heterologous expression of TdCBL6 in Arabidopsis confers salt tolerance by reducing membrane injury and improving photosynthetic efficiency, and that the TdCBL6 gene may be involved in response to LK stress by regulating the reactive oxygen species-mediated LK signaling pathway.