RUNX3 expression is associated with sensitivity to pheophorbide a-based photodynamic therapy in keloids

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• 作者：Zhenlong Zheng (1)
  Lianhua Zhu (1)
  Xianglan Zhang (3) (4)
  Lianhua Li (1)
  Sook Moon (4)
  Mi Ryung Roh (2)
  Zhehu Jin (1)
  
  1. Department of Dermatology ; Yanbian University Hospital ; Yanji City ; Jilin Province ; China
  3. Department of Pathology ; Yanbian University Hospital ; Yanji City ; Jilin Province ; China
  4. Oral Cancer Research Institute ; College of Dentistry ; Yonsei University ; Seoul ; South Korea
  2. Department of Dermatology ; Yonsei University College of Medicine ; Seoul ; South Korea
  
• 关键词：RUNX3 ; Pheophorbide a ; based photodynamic therapy ; Keloid
• 刊名：Lasers in Medical Science
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：30
• 期：1
• 页码：67-75
• 全文大小：3,543 KB
• 参考文献：1. Mrowietz U, Seifert O (2009) Keloid scarring: new treatments ahead. Actas Dermosifiliogr 100(Suppl 2):75鈥?3 CrossRef
  2. Shih B, Bayat A (2010) Genetics of keloid scarring. Arch Dermatol Res 302(5):319鈥?39 CrossRef
  3. Calderon M, Lawrence WT, Banes AJ (1996) Increased proliferation in keloid fibroblasts wounded in vitro. J Surg Res 61(2):343鈥?47 CrossRef
  4. Blazic TM, Brajac I (2006) Defective induction of senescence during wound healing is a possible mechanism of keloid formation. Med Hypotheses 66(3):649鈥?52 CrossRef
  5. Bock O, Schmid-Ott G, Malewski P, Mrowietz U (2006) Quality of life of patients with keloid and hypertrophic scarring. Arch Dermatol Res 297(10):433鈥?38 CrossRef
  6. Shaffer JJ, Taylor SC, Cook-Bolden F (2002) Keloidal scars: a review with a critical look at therapeutic options. J Am Acad Dermatol 46(2 Suppl Understanding):S63鈥揝97 CrossRef
  7. Niessen FB, Spauwen PH, Schalkwijk J, Kon M (1999) On the nature of hypertrophic scars and keloids: a review. Plast Reconstr Surg 104(5):1435鈥?458 CrossRef
  8. Kelly AP (2004) Medical and surgical therapies for keloids. Dermatol Ther 17(2):212鈥?18 CrossRef
  9. Mall JW, Pollmann C, Muller JM, Buttemeyer R (2002) Keloid of the earlobe after ear piercing. Not only a surgical problem. Chirurg 73(5):514鈥?16 CrossRef
  10. Durani P, Bayat A (2008) Levels of evidence for the treatment of keloid disease. J Plast Reconstr Aesthet Surg 61(1):4鈥?7 CrossRef
  11. Christensen E, Warloe T, Kroon S, Funk J, Helsing P, Soler AM et al (2010) Guidelines for practical use of MAL-PDT in non-melanoma skin cancer. J Eur Acad Dermatol Venereol 24(5):505鈥?12 CrossRef
  12. Dolmans DE, Fukumura D, Jain RK (2003) Photodynamic therapy for cancer. Nat Rev Cancer 3(5):380鈥?87 CrossRef
  13. Wilson BC (2002) Photodynamic therapy for cancer: principles. Can J Gastroenterol 16(6):393鈥?96
  14. Vrouenraets MB, Visser GW, Snow GB, van Dongen GA (2003) Basic principles, applications in oncology and improved selectivity of photodynamic therapy. Anticancer Res 23(1B):505鈥?22
  15. Brown SB, Brown EA, Walker I (2004) The present and future role of photodynamic therapy in cancer treatment. Lancet Oncol 5(8):497鈥?08 CrossRef
  16. Luna MC, Ferrario A, Wong S, Fisher AM, Gomer CJ (2000) Photodynamic therapy-mediated oxidative stress as a molecular switch for the temporal expression of genes ligated to the human heat shock promoter. Cancer Res 60(6):1637鈥?644
  17. Lim DS, Ko SH, Lee WY (2004) Silkworm-pheophorbide alpha mediated photodynamic therapy against B16F10 pigmented melanoma. J Photochem Photobiol, B 74(1):1鈥? CrossRef
  18. Tang PM, Liu XZ, Zhang DM, Fong WP, Fung KP (2009) Pheophorbide a based photodynamic therapy induces apoptosis via mitochondrial-mediated pathway in human uterine carcinosarcoma. Cancer Biol Ther 8(6):533鈥?39 CrossRef
  19. Hoi SW, Wong HM, Chan JY, Yue GG, Tse GM, Law BK et al (2012) Photodynamic therapy of pheophorbide a inhibits the proliferation of human breast tumour via both caspase-dependent and -independent apoptotic pathways in in vitro and in vivo models. Phytother Res 26(5):734鈥?42 CrossRef
  20. Tang PM, Chan JY, Au SW, Kong SK, Tsui SK, Waye MM et al (2006) Pheophorbide a, an active compound isolated from Scutellaria barbata, possesses photodynamic activities by inducing apoptosis in human hepatocellular carcinoma. Cancer Biol Ther 5(9):1111鈥?116 CrossRef
  21. Hajri A, Wack S, Meyer C, Smith MK, Leberquier C, Kedinger M et al (2002) In vitro and in vivo efficacy of photofrin and pheophorbide a, a bacteriochlorin, in photodynamic therapy of colonic cancer cells. Photochem Photobiol 75(2):140鈥?48 CrossRef
  22. Lee WY, Lim DS, Ko SH, Park YJ, Ryu KS, Ahn MY et al (2004) Photoactivation of pheophorbide a induces a mitochondrial-mediated apoptosis in Jurkat leukaemia cells. J Photochem Photobiol, B 75(3):119鈥?26 CrossRef
  23. Moon S, Bae JY, Son HK, Lee DY, Park G, You H et al (2013) RUNX3 confers sensitivity to pheophorbide a-photodynamic therapy in human oral squamous cell carcinoma cell lines. Lasers Med Sci. doi:10.1007/s10103-013-1350-1
  24. Ito K (2011) RUNX3 in oncogenic and anti-oncogenic signaling in gastrointestinal cancers. J Cell Biochem 112(5):1243鈥?249 CrossRef
  25. Lee CW, Chuang LS, Kimura S, Lai SK, Ong CW, Yan B et al (2011) RUNX3 functions as an oncogene in ovarian cancer. Gynecol Oncol 122(2):410鈥?17 CrossRef
  26. Kudo Y, Tsunematsu T, Takata T (2011) Oncogenic role of RUNX3 in head and neck cancer. J Cell Biochem 112(2):387鈥?93 CrossRef
  27. Iqbal SA, Syed F, McGrouther DA, Paus R, Bayat A (2010) Differential distribution of haematopoietic and nonhaematopoietic progenitor cells in intralesional and extralesional keloid: do keloid scars provide a niche for nonhaematopoietic mesenchymal stem cells? Br J Dermatol 162(6):1377鈥?383 CrossRef
  28. Lau K, Paus R, Tiede S, Day P, Bayat A (2009) Exploring the role of stem cells in cutaneous wound healing. Exp Dermatol 18(11):921鈥?33 CrossRef
  29. Vincent AS, Phan TT, Mukhopadhyay A, Lim HY, Halliwell B, Wong KP (2008) Human skin keloid fibroblasts display bioenergetics of cancer cells. J Invest Dermatol 128(3):702鈥?09 CrossRef
  30. Zhang G, Jiang JJ, Luo SJ, Tang SM, Liang J, Yu Q (2008) The relationship between RUNX3 gene mutation and keloid. Zhonghua Zheng Xing Wai Ke Za Zhi 24(3):224鈥?27
  31. Luo X, Pan Q, Liu L, Chegini N (2007) Genomic and proteomic profiling II: comparative assessment of gene expression profiles in leiomyomas, keloids, and surgically-induced scars. Reprod Biol Endocrinol 5:35 CrossRef
  32. Ahn MY, Kwon SM, Kim YC, Ahn SG, Yoon JH (2012) Pheophorbide a-mediated photodynamic therapy induces apoptotic cell death in murine oral squamous cell carcinoma in vitro and in vivo. Oncol Rep 27(6):1772鈥?778
  33. Lim SH, Lee HB, Ho AS (2011) A new naturally derived photosensitizer and its phototoxicity on head and neck cancer cells. Photochem Photobiol 87(5):1152鈥?158 CrossRef
  34. Mendoza J, Sebastian A, Allan E, Allan D, Mandal P, Alonso-Rasgado T et al (2012) Differential cytotoxic response in keloid fibroblasts exposed to photodynamic therapy is dependent on photosensitiser precursor, fluence and location of fibroblasts within the lesion. Arch Dermatol Res 304(7):549鈥?62 CrossRef
  35. Chiu LL, Sun CH, Yeh AT, Torkian B, Karamzadeh A, Tromberg B et al (2005) Photodynamic therapy on keloid fibroblasts in tissue-engineered keratinocyte-fibroblast co-culture. Lasers Surg Med 37(3):231鈥?44 CrossRef
  36. Nie Z, Bayat A, Behzad F, Rhodes LE (2010) Positive response of a recurrent keloid scar to topical methyl aminolevulinate-photodynamic therapy. Photodermatol Photoimmunol Photomed 26(6):330鈥?32 CrossRef
  37. Hsieh YJ, Wu CC, Chang CJ, Yu JS (2003) Subcellular localization of Photofrin determines the death phenotype of human epidermoid carcinoma A431 cells triggered by photodynamic therapy: when plasma membranes are the main targets. J Cell Physiol 194(3):363鈥?75 CrossRef
  38. Almeida RD, Manadas BJ, Carvalho AP, Duarte CB (2004) Intracellular signaling mechanisms in photodynamic therapy. Biochim Biophys Acta 1704(2):59鈥?6
  39. Salto-Tellez M, Peh BK, Ito K, Tan SH, Chong PY, Han HC et al (2006) RUNX3 protein is overexpressed in human basal cell carcinomas. Oncogene 25(58):7646鈥?649 CrossRef
  40. Tsunematsu T, Kudo Y, Iizuka S, Ogawa I, Fujita T, Kurihara H et al (2009) RUNX3 has an oncogenic role in head and neck cancer. PLoS One 4(6):e5892 CrossRef
  41. Nevadunsky NS, Barbieri JS, Kwong J, Merritt MA, Welch WR, Berkowitz RS et al (2009) RUNX3 protein is overexpressed in human epithelial ovarian cancer. Gynecol Oncol 112(2):325鈥?30 CrossRef
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Medicine/Public Health, general
  Dentistry
  Laser Technology and Physics and Photonics
  Quantum Optics, Quantum Electronics and Nonlinear Optics
  Applied Optics, Optoelectronics and Optical Devices
  
• 出版者：Springer London
• ISSN：1435-604X

文摘

Runt-related transcription factor 3 (RUNX3) has recently been reported to be a possible predictor of sensitivity of cancer cells for photodynamic therapy (PDT), a promising therapeutic modality for keloids. In this study, we aimed to elucidate the implications of RUNX3 for keloid pathogenesis and sensitivity to pheophorbide a-based PDT (Pa-PDT). RUNX3 and proliferating cell nuclear antigen (PCNA) expression were examined in 6 normal skin samples and 32 keloid tissue samples by immunohistochemistry. We found that RUNX3 expression was detected more often in keloid tissues than in dermis of normal skin. In keloid tissues, RUNX3 expression was significantly increased in patients presenting with symptoms of pain or pruritus, and was also significantly related to PCNA expression. The therapeutic effect of Pa-PDT was comparatively investigated in keloid fibroblasts (KFs) with and without RUNX3 expression. Significant differences were found after Pa-PDT between KFs with and without RUNX3 expression in cell viability, proliferative ability, type I collagen expression, generation of reactive oxygen species (ROS), and apoptotic cell death. In addition, RUNX3 expression was significantly decreased after Pa-PDT in KFs, and KFs with downregulation of RUNX3 showed significantly increased cell viability after Pa-PDT. Pa-PDT may be a potential therapeutic modality for keloids, and RUNX3, as a possible contributor to keloid pathogenesis, may improve sensitivity to Pa-PDT in KFs.