Comparative analysis of diploid species of Avena L. using cytogenetic and biochemical markers: Avena canariensis Baum et Fedak and A. longiglumis Dur.

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• 作者：O. Yu. Shelukhina ; E. D. Badaeva ; T. A. Brezhneva ; I. G. Loskutov and V. A. Pukhalsky
• 刊名：Russian Journal of Genetics
• 出版年：2008
• 出版时间：June, 2008
• 年：2008
• 卷：44
• 期：6
• 页码：694-701
• 全文大小：362.1 KB

文摘

The diploid oat species containing the A genome of two types (Al and Ac) were studied by electrophoresis of grain storage proteins (avenins), chromosome C-banding, and in situ hybridization with probes pTa71 and pTa794. The karyotypes of the studied species displayed similar C-banding patterns but differed in size and morphology of several chromosomes, presumably, resulting from structural rearrangements that took place during the divergence of A genomes from a common ancestor. In situ hybridization demonstrated an identical location of the 45S and 5S rRNA gene loci in Avena canariensis and A. longiglumis similar to that in the A. strigosa genome. However, the 5S rDNA locus in A. longiglumis (5S rDNA1) was considerably decreased in the chromosome 3Al long arm. The analysis demonstrated that these oat species were similar in the avenin component composition, although individual accessions differed in the electrophoretic mobilities of certain components. A considerable similarity of A. canariensis and A. longiglumis to the Avena diploid species carrying the As genome variant was demonstrated.