Association of six YFP-myosin XI-tail fusions with mobile plant cell organelles

详细信息    查看全文

• 作者：Daniel Reisen (1) (2)
  Maureen R Hanson (1)
  
• 刊名：BMC Plant Biology
• 出版年：2007
• 出版时间：December 2007
• 年：2007
• 卷：7
• 期：1
• 全文大小：7528KB
• 参考文献：1. Logan DC, Leaver CJ: ass="a-plus-plus">Mitochondria-targeted GFP highlights the heterogeneity of mitochondrial shape, size and movement within living plant cells. / J Exp Bot 2000, ass="a-plus-plus">51:865-71. <a class="external" href="http://dx.doi.org/10.1093/jexbot/51.346.865">CrossRefa>
  2. K?hler RH, Zipfel WR, Webb WW, Hanson MR: ass="a-plus-plus">The green fluorescent protein as a marker to visualize plant mitochondria in vivo. / Plant J 1997, ass="a-plus-plus">11:613-21. <a class="external" href="http://dx.doi.org/10.1046/j.1365-313X.1997.11030613.x">CrossRefa>
  3. Wada M, Kagawa T, Sato Y: ass="a-plus-plus">Chloroplast movement. / Annual Review of Plant Biology 2003, ass="a-plus-plus">54:455-68. <a class="external" href="http://dx.doi.org/10.1146/annurev.arplant.54.031902.135023">CrossRefa>
  4. Kwok EY, Hanson MR: ass="a-plus-plus">Stromules and the dynamic nature of plastid morphology. / J Microsc 2004, ass="a-plus-plus">214:124-37. <a class="external" href="http://dx.doi.org/10.1111/j.0022-2720.2004.01317.x">CrossRefa>
  5. Jedd G, Chua NH: ass="a-plus-plus">Visualization of peroxisomes in living plant cells reveals acto-myosin-dependent cytoplasmic streaming and peroxisome budding. / Plant Cell Physiol 2002, ass="a-plus-plus">43:384-92. <a class="external" href="http://dx.doi.org/10.1093/pcp/pcf045">CrossRefa>
  6. Collings DA, Harper JDI, Marc J, Overall RL, Mullen RT: ass="a-plus-plus">Life in the fast lane: actin-based motility of plant peroxisomes. / Canadian Journal of Botany 2002, ass="a-plus-plus">80:430-41. <a class="external" href="http://dx.doi.org/10.1139/b02-036">CrossRefa>
  7. Nebenführ A, Gallagher LA, Dunahay TG, Frohlick JA, Mazurkiewicz AM, Meehl JB, Staehelin LA: ass="a-plus-plus">Stop-and-go movements of plant golgi stacks are mediated by the acto-myosin system. / Plant Physiol 1999, ass="a-plus-plus">121:1127-141. <a class="external" href="http://dx.doi.org/10.1104/pp.121.4.1127">CrossRefa>
  8. Van Gestel K, Kohler RH, Verbelen JP: ass="a-plus-plus">Plant mitochondria move on F-actin, but their positioning in the cortical cytoplasm depends on both F-actin and microtubules. / J Exp Bot 2002, ass="a-plus-plus">53:659-67. <a class="external" href="http://dx.doi.org/10.1093/jexbot/53.369.659">CrossRefa>
  9. Sato Y, Wada M, Kadota A: ass="a-plus-plus">Choice of tracks, microtubules and/or actin filaments for chloroplast photo-movement is differentially controlled by phytochrome and a blue light receptor. / J Cell Sci 2001, ass="a-plus-plus">114:269-79.
  10. Kwok EY, Hanson MR: ass="a-plus-plus">Microfilaments and microtubules control the morphology and movement of non-green plastids and stromules in Nicotiana tabacum. / Plant J 2003, ass="a-plus-plus">35:16-6. <a class="external" href="http://dx.doi.org/10.1046/j.1365-313X.2003.01777.x">CrossRefa>
  11. Sellers JR: ass="a-plus-plus">Myosins: a diverse superfamily. / Biochimica et Biophysica Acta 2000, ass="a-plus-plus">1496:3-2.
  12. Berg JS, Powell BC, Cheney RE: ass="a-plus-plus">A millennial myosin census. / Mol Biol Cell 2001, ass="a-plus-plus">12:780-94.
  13. Kieke MC, Titus MA: ass="a-plus-plus">Myosin superfamily: An overview. / Molecular motors / (Edited by: Schliwa M). , Wiley-VCH, Germany 2003, 3-4.
  14. Burkhard P, Stetefeld J, Strelkov SV: ass="a-plus-plus">Coiled coils: a highly versatile protein folding motif. / Trends in Cell Biology 2001, ass="a-plus-plus">11:82-8. <a class="external" href="http://dx.doi.org/10.1016/S0962-8924(00)01898-5">CrossRefa>
  15. Reddy ASN, Day IS: ass="a-plus-plus">Analysis of the myosins encoded in the recently completed Arabidopsis thaliana genome sequence. / Genome Biology 2001, ass="a-plus-plus">2:research0024.1 - research0024.17. <a class="external" href="http://dx.doi.org/10.1186/gb-2001-2-7-research0024">CrossRefa>
  16. Reddy ASN: ass="a-plus-plus">Molecular motors and their functions in plants. / International Review of Cytology 2001, ass="a-plus-plus">204:97-78. <a class="external" href="http://dx.doi.org/10.1016/S0074-7696(01)04004-9">CrossRefa>
  17. Bezanilla M, Horton AC, Sevener HC, Quatrano RS: ass="a-plus-plus">Phylogenetic analysis of new plant myosin sequences. / J Mol Evol 2003, ass="a-plus-plus">57:229-39. <a class="external" href="http://dx.doi.org/10.1007/s00239-003-2469-7">CrossRefa>
  18. Jiang SY, Ramachandran S: ass="a-plus-plus">Identification and molecular characterization of myosin gene family in Oryza sativa genome. / Plant Cell Physiol 2004, ass="a-plus-plus">45:590-99. <a class="external" href="http://dx.doi.org/10.1093/pcp/pch061">CrossRefa>
  19. Reichelt S, Knight AE, Hodge TP, Baluska F, Samaj J, Volkmann D, Kendrick-Jones J: ass="a-plus-plus">Characterization of the unconventional myosin VIII in plant cells and its localization at the post-cytokinetic cell wall. / Plant J 1999, ass="a-plus-plus">19:555-67. <a class="external" href="http://dx.doi.org/10.1046/j.1365-313X.1999.00553.x">CrossRefa>
  20. Kinkema M, Wang H, Schiefelbein J: ass="a-plus-plus">Molecular analysis of the myosin gene family in Arabidopsis thaliana. / Plant Molecular Biology 1994, ass="a-plus-plus">26:1139-153. <a class="external" href="http://dx.doi.org/10.1007/BF00040695">CrossRefa>
  21. Au JSY, Huang JD: ass="a-plus-plus">A tissue-specific exon of myosin Va is responsible for selective cargo binding in melanocytes. / Cell Motility and the Cytoskeleton 2002, ass="a-plus-plus">53:89-02. <a class="external" href="http://dx.doi.org/10.1002/cm.10061">CrossRefa>
  22. Boevink P, Oparka K, Cruz SS, Martin B, Betteridge A, Hawes C: ass="a-plus-plus">Stacks on tracks: the plant Golgi apparatus traffics on an actin/ER network. / The Plant Journal 1998, ass="a-plus-plus">15:441-47. <a class="external" href="http://dx.doi.org/10.1046/j.1365-313X.1998.00208.x">CrossRefa>
  23. Gould SJ, Keller GA, Hosken N, Wilkinson J, Subramani S: ass="a-plus-plus">A conserved tripeptide sorts proteins to peroxisomes. / J Cell Biol 1989, ass="a-plus-plus">108:1657-664. <a class="external" href="http://dx.doi.org/10.1083/jcb.108.5.1657">CrossRefa>
  24. Kamigaki A, Mano S, Terauchi K, Nishi Y, Tachibe-Kinoshita Y, Nito K, Kondo M, Hayashi M, Nishimura M, Esaka M: ass="a-plus-plus">Identification of peroxisomal targeting signal of pumpkin catalase and the binding analysis with PTS1 receptor. / The Plant Journal 2003, ass="a-plus-plus">33:161-75. <a class="external" href="http://dx.doi.org/10.1046/j.0960-7412.2003.001605.x">CrossRefa>
  25. Mano S, Nakamori C, Hayashi M, Kato A, Kondo M, Nishimura M: ass="a-plus-plus">Distribution and characterization of peroxisomes in Arabidopsis by visualization with GFP: Dynamic morphology and actin-dependent movement. / Plant Cell Physiol 2002, ass="a-plus-plus">43:331-41. <a class="external" href="http://dx.doi.org/10.1093/pcp/pcf037">CrossRefa>
  26. Wu X, Bowers B, Rao K, Wei Q, Hammer JA 3rd: ass="a-plus-plus">Visualization of melanosome dynamics within wild-type and dilute melanocytes suggests a paradigm for myosin V function In vivo. / J Cell Biol 1998, ass="a-plus-plus">143:1899-918. <a class="external" href="http://dx.doi.org/10.1083/jcb.143.7.1899">CrossRefa>
  27. Gross SP, Tuma MC, Deacon SW, Serpinskaya AS, Reilein AR, Gelfand VI: ass="a-plus-plus">Interactions and regulation of molecular motors in Xenopus melanophores. / J Cell Biol 2002, ass="a-plus-plus">156:855-65. <a class="external" href="http://dx.doi.org/10.1083/jcb.200105055">CrossRefa>
  28. Lu L, Lee YRJ, Pan R, Maloof JN, Liu B: ass="a-plus-plus">An internal motor kinesin is associated with the Golgi apparatus and plays a role in trichome morphogenesis in Arabidopsis. / Mol Biol Cell 2005, ass="a-plus-plus">16:811-23. <a class="external" href="http://dx.doi.org/10.1091/mbc.E04-05-0400">CrossRefa>
  29. Levi V, Serpinskaya AS, Gratton E, Gelfand V: ass="a-plus-plus">Organelle transport along microtubules in Xenopus melanophores: evidence for cooperation between multiple motors. / Biophys J 2006, ass="a-plus-plus">90:318-27. <a class="external" href="http://dx.doi.org/10.1529/biophysj.105.067843">CrossRefa>
  30. ass="a-plus-plus">Pfam[<a href="http://www.sanger.ac.uk/Software/Pfam/search.shtml" class="a-plus-plus">http://www.sanger.ac.uk/Software/Pfam/search.shtmla>]
  31. Hashimoto K, Igarashi H, Mano S, Nishimura M, Shimmen T, Yokota E: ass="a-plus-plus">Peroxisomal localization of a myosin XI isoform in Arabidopsis thaliana. / Plant Cell Physiol 2005, ass="a-plus-plus">46:782-89. <a class="external" href="http://dx.doi.org/10.1093/pcp/pci085">CrossRefa>
  32. Voigt B, Timmers ACJ, Samaj J, Muller J, Baluska F, Menzel D: ass="a-plus-plus">GFP-FABD2 fusion construct allows in vivo visualization of the dynamic actin cytoskeleton in all cells of Arabidopsis seedlings. / European Journal of Cell Biology 2005, ass="a-plus-plus">84:595-08. <a class="external" href="http://dx.doi.org/10.1016/j.ejcb.2004.11.011">CrossRefa>
  33. Kost B, Spielhofer P, Chua NH: ass="a-plus-plus">A GFP-mouse talin fusion protein labels plant actin filaments in vivo and visualizes the actin cytoskeleton in growing pollen tubes. / The Plant Journal 1998, ass="a-plus-plus">16:393-01. <a class="external" href="http://dx.doi.org/10.1046/j.1365-313x.1998.00304.x">CrossRefa>
  34. Marc J, Granger CL, Brincat J, Fisher DD, Kao T, McCubbin AG, Cyr RJ: ass="a-plus-plus">A GFP–MAP4 reporter gene for visualizing cortical microtubule rearrangements in living epidermal cells. / Plant Cell 1998, ass="a-plus-plus">10:1927-940. <a class="external" href="http://dx.doi.org/10.1105/tpc.10.11.1927">CrossRefa>
  35. Lantz VA, Miller KG: ass="a-plus-plus">A class VI unconventional myosin is associated with a homologue of a microtubule-binding protein, cytoplasmic linker protein-170, in neurons and at the posterior pole of Drosophila embryos. / J Cell Biol 1998, ass="a-plus-plus">140:897-10. <a class="external" href="http://dx.doi.org/10.1083/jcb.140.4.897">CrossRefa>
  36. Schliwa M, Woehlke G: ass="a-plus-plus">Molecular motors. / Nature 2003, ass="a-plus-plus">422:759-65. <a class="external" href="http://dx.doi.org/10.1038/nature01601">CrossRefa>
  37. Mathur J, Mathur N, Hulskamp M: ass="a-plus-plus">Simultaneous visualization of peroxisomes and cytoskeletal elements reveals actin and not microtubule-based peroxisome motility in plants. / Plant Physiol 2002, ass="a-plus-plus">128:1031-045. <a class="external" href="http://dx.doi.org/10.1104/pp.011018">CrossRefa>
  38. Chuong S, Park NI, Freeman M, Mullen R, Muench D: ass="a-plus-plus">The peroxisomal multifunctional protein interacts with cortical microtubules in plant cells. / BMC Cell Biology 2005, ass="a-plus-plus">6:40. <a class="external" href="http://dx.doi.org/10.1186/1471-2121-6-40">CrossRefa>
  39. Rapp S, Saffrich R, Anton M, Jakle U, Ansorge W, Gorgas K, Just WW: ass="a-plus-plus">Microtubule-based peroxisome movement. / J Cell Sci 1996, ass="a-plus-plus">109:837-49.
  40. Earley KW, Haag JR, Pontes O, Opper K, Juehne T, Song K, Pikaard CS: ass="a-plus-plus">Gateway-compatible vectors for plant functional genomics and proteomics. / The Plant Journal 2006, ass="a-plus-plus">45:616-29. <a class="external" href="http://dx.doi.org/10.1111/j.1365-313X.2005.02617.x">CrossRefa>
  41. Goodin MM, Dietzgen RG, Schichnes D, Ruzin S, Jackson AO: ass="a-plus-plus">pGD vectors: versatile tools for the expression of green and red fluorescent protein fusions in agroinfiltrated plant leaves. / Plant J 2002, ass="a-plus-plus">31:375-83. <a class="external" href="http://dx.doi.org/10.1046/j.1365-313X.2002.01360.x">CrossRefa>
  42. Corpet F: ass="a-plus-plus">Multiple sequence alignment with hierarchical clustering. / Nucl Acids Res 1988, ass="a-plus-plus">16:10881-0890. <a class="external" href="http://dx.doi.org/10.1093/nar/16.22.10881">CrossRefa>
  43. ass="a-plus-plus">GeneDoc[<a href="http://www.psc.edu/biomed/genedoc/" class="a-plus-plus">http://www.psc.edu/biomed/genedoc/a>]
  44. Lupas A, Van Dyke M, Stock J: ass="a-plus-plus">Predicting coiled coils from protein sequences. / Science 1991, ass="a-plus-plus">252:1162-164. <a class="external" href="http://dx.doi.org/10.1126/science.252.5009.1162">CrossRefa>
  45. Schultz J, Milpetz F, Bork P, Ponting CP: ass="a-plus-plus">SMART, a simple modular architecture research tool: Identification of signaling domains. / PNAS 1998, ass="a-plus-plus">95:5857-864. <a class="external" href="http://dx.doi.org/10.1073/pnas.95.11.5857">CrossRefa>
  46. Letunic I, Copley RR, Pils B, Pinkert S, Schultz J, Bork P: ass="a-plus-plus">SMART 5: domains in the context of genomes and networks. / Nucl Acids Res 2006, ass="a-plus-plus">34:D257-60. <a class="external" href="http://dx.doi.org/10.1093/nar/gkj079">CrossRefa>
  47. Bendahmane A, Farnham G, Moffett P, Baulcombe DC: ass="a-plus-plus">Constitutive gain-of-function mutants in a nucleotide binding site-leucine rich repeat protein encoded at the Rx locus of potato. / The Plant Journal 2002, ass="a-plus-plus">32:195-04. <a class="external" href="http://dx.doi.org/10.1046/j.1365-313X.2002.01413.x">CrossRefa>
  48. Hamilton CM, Frary A, Lewis C, Tanksley SD: ass="a-plus-plus">Stable transfer of intact high molecular weight DNA into plant chromosomes. / PNAS 1996, ass="a-plus-plus">93:9975-979. <a class="external" href="http://dx.doi.org/10.1073/pnas.93.18.9975">CrossRefa>
  
• 作者单位：Daniel Reisen (1) (2)
  Maureen R Hanson (1)
  
  1. Department of Molecular Biology and Genetics, 321 Biotechnology Building, Cornell University, Ithaca, NY, 14853, USA
  2. Bitplane AG, Badenerstrasse 682, CH-8048, Zurich, Switzerland
  

文摘

Background Myosins are molecular motors that carry cargo on actin filaments in eukaryotic cells. Seventeen myosin genes have been identified in the nuclear genome of Arabidopsis. The myosin genes can be divided into two plant-specific subfamilies, class VIII with four members and class XI with 13 members. Class XI myosins are related to animal and fungal myosin class V that are responsible for movement of particular vesicles and organelles. Organelle localization of only one of the 13 Arabidopsis myosin XI (myosin XI-6; At MYA2), which is found on peroxisomes, has so far been reported. Little information is available concerning the remaining 12 class XI myosins. Results We investigated 6 of the 13 class XI Arabidopsis myosins. cDNAs corresponding to the tail region of 6 myosin genes were generated and incorporated into a vector to encode YFP-myosin tail fusion proteins lacking the motor domain. Chimeric genes incorporating tail regions of myosin XI-5 (At MYA1), myosin XI-6 (At MYA2), myosin XI-8 (At XI-B), myosin XI-15 (At XI-I), myosin XI-16 (At XI-J) and myosin XI-17 (At XI-K) were expressed transiently. All YFP-myosin-tail fusion proteins were targeted to small organelles ranging in size from 0.5 to 3.0 μm. Despite the absence of a motor domain, the fluorescently-labeled organelles were motile in most cells. Tail cropping experiments demonstrated that the coiled-coil region was required for specific localization and shorter tail regions were inadequate for targeting. Myosin XI-6 (At MYA2), previously reported to localize to peroxisomes by immunofluorescence, labeled both peroxisomes and vesicles when expressed as a YFP-tail fusion. None of the 6 YFP-myosin tail fusions interacted with chloroplasts, and only one YFP-tail fusion appeared to sometimes co-localize with fluorescent proteins targeted to Golgi and mitochondria. Conclusion 6 myosin XI tails, extending from the coiled-coil region to the C-terminus, label specific vesicles and/or organelles when transiently expressed as YFP fusions in plant cells. Although comparable constructs lacking the motor domain result in a dominant negative effect on organelle motility in animal systems, the plant organelles remained motile. YFP-myosin tail fusions provide specific labeling for vesicles of unknown composition, whose identity can be investigated in future studies.