Nano-Au/cMWCNT Modified speB Gene Specific Amperometric Sensor for Rapidly Detecting Streptococcus pyogenes causing Rheumatic Heart Disease

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• 作者：Ankur Kaushal ; Swati Singh ; Ashok Kumar ; Dinesh Kumar
• 关键词：Nano ; Au/cMWCNT ; speB sensor ; Rheumatic heart disease ; Streptococcus pyogenes
• 刊名：Indian Journal of Microbiology
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：57
• 期：1
• 页码：121-124
• 全文大小：
• 刊物主题：Microbiology; Medical Microbiology;
• 出版者：Springer India
• ISSN：0973-7715
• 卷排序：57

文摘

A specific 5′ NH₂ labeled DNA probe of speB gene was immobilized onto the gold nanoparticles/carboxylated multi walled carbon nanotubes (Nano-Au/cMWCNT) screen printed electrode using EDC/NHS cross linking chemistry. This was followed by hybridization with 0.5–50 ng/6 µl of single stranded genomic DNA Streptococcus pyogenes infected patient throat swab samples. Electrochemical amperometric assay was deciphered by using cyclic voltammetry (CV) with methylene blue a redox indicator. The sensor had a sensitivity of 104.7 µA cm−2 ng−1 using CV with a R2 of 0.907 and 0.01 ng/6 µl as the limit of detection (LOD). The modified electrode surface morphology was characterized using scanning electron microscopy. The stability of the electrode was seen at 4 °C for 180 days having 6% loss in the initial current. The sensor is speB gene specific and can detect the pathogen within 30 min.