Genetic tools to support the conservation of the endangered smalltooth sawfish, Pristis pectinata
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- 作者：Kevin A. Feldheim (1)
Demian D. Chapman (2) (3)
Colin A. Simpfendorfer (4)
Vincent P. Richards (5)
Mahmood S. Shivji (5)
Tonya R. Wiley (6)
Gregg R. Poulakis (7)
John K. Carlson (8)
Rowena Eng (3)
Skyler Sagarese (3) - 关键词：Sawfish ; Microsatellites ; DNA barcoding ; Wildlife trade
- 刊名：Conservation Genetics Resources
- 出版年：2010
- 出版时间：December 2010
- 年：2010
- 卷：2
- 期：1
- 页码：105-113
- 全文大小：297KB
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Demian D. Chapman (2) (3)
Colin A. Simpfendorfer (4)
Vincent P. Richards (5)
Mahmood S. Shivji (5)
Tonya R. Wiley (6)
Gregg R. Poulakis (7)
John K. Carlson (8)
Rowena Eng (3)
Skyler Sagarese (3)
1. Pritzker Laboratory for Molecular Systematics and Evolution, The Field Museum, 1400 S. Lake Shore Drive, Chicago, IL, 60605, USA
2. Institute for Ocean Conservation Science, Stony Brook University, Stony Brook, NY, 11794-5000, USA
3. School of Marine and Atmospheric Science, Stony Brook University, Stony Brook, NY, 11794-5000, USA
4. Fishing and Fisheries Research Centre, School of Earth and Environmental Sciences, James Cook University, Townsville, QLD, 4811, Australia
5. The Guy Harvey Research Institute, Oceanographic Center, Nova Southeastern University, Dania Beach, FL, 33004, USA
6. Texas Parks and Wildlife Department, Coastal Fisheries Division, Dickinson Marine Lab, Dickinson, TX, 77539, USA
7. Florida Fish and Wildlife Conservation Commission, Fish and Wildlife Research Institute, Charlotte Harbor Field Laboratory, 585 Prineville Street, Port Charlotte, FL, 33954-1006, USA
8. NOAA Fisheries Service, Southeast Fisheries Science Center, 3500 Delwood Beach Rd, Panama City, FL, 32408, USA
文摘
The smalltooth sawfish, Pristis pectinata, is protected under the US Endangered Species Act (ESA) and all forms of international trade of this species are prohibited under Appendix I of the Convention on International Trade in Endangered Species of Flora and Fauna (CITES). Although it is illegal to land or trade P. pectinata within the US or across its borders, it is difficult to enforce these regulations for some sawfish body parts because they resemble legally-traded shark body parts (e.g. dried fins). There is also a growing need for conservation genetics research on this species and its relatives, including assessments of population structure and genetic diversity. Given these pressing trade monitoring and research needs, we developed: (1) a rapid PCR-based test to identify P. pectinata body parts in trade in the US and western Atlantic, (2) a DNA-barcode based on 520?bp of cytochrome b that resolves P. pectinata and five other extant sawfish species and (3) a suite of 11 polymorphic P. pectinata microsatellite markers that can be used in a variety of conservation genetics applications for this and other sawfish species. We anticipate that this suite of genetic tools will contribute to the conservation of this critically endangered species and its relatives by reinforcing landings and trade restrictions and by enabling future conservation genetics research.