Attenuated macrophage cholesterol efflux function in patients with obstructive sleep apnea–hypopnea syndrome

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• 作者：Rui-Yi Xu ; Rong Huang ; Yi Xiao ; Lian-Feng Chen ; Xue Lin ; Quan Fang…
• 关键词：Macrophage ; ATP ; binding cassette transporter protein A1 ; Cholesterol efflux ; Obstructive sleep apnea–hypopnea syndrome
• 刊名：Sleep and Breathing
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：19
• 期：1
• 页码：369-375
• 全文大小：440 KB
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• 刊物主题：Pneumology/Respiratory System; Otorhinolaryngology; Dentistry; Neurology; Internal Medicine; Pediatrics;
• 出版者：Springer Berlin Heidelberg
• ISSN：1522-1709

文摘

Background Obstructive sleep apnea–hypopnea syndrome (OSAHS) is associated with premature atherosclerosis. However, the associated mechanism remains unknown. This study investigates the expression of adenosine triphosphate (ATP)-binding cassette transporter protein A1 (ABCA1) and cellular cholesterol efflux in cultured macrophages from OSAHS patients. Methods Of the 18 subjects enrolled in this study, six subjects with apnea–hypopnea index (AHI) mRNA and protein expression were evaluated by reverse transcription PCR and Western Blot, respectively. Both ABCA1-mediated and autologous serum induced cholesterol efflux were measured by isotopic cholesterol efflux assays. Results The levels of AHI and high sensitivity C-reactive protein (hsCRP) were significantly higher in the OSAHS group than in the control group. ABCA1 mRNA and protein expressions in PBMCs-derived macrophages were significantly reduced in patients with OSAHS compared to that in controls (p--.05). Both ABCA1-mediated and autologous serum-induced cholesterol efflux were significantly lower in the OSAHS group than that in the control group (p--.033 and p--.01, respectively). Pearson’s correlation analysis revealed a negative correlation between AHI and the mRNA (r-??0.7726, p--.0007) and protein (r-??0.8112, p--.0044) expression of ABCA1, a positive correlation between ABCA1-mediated cholesterol efflux and the minimum oxygen saturation (r--.7954, p--.0001), and a negative correlation between AHI and autologous serum induced cholesterol efflux (r-??0.7756, p--.0002). Conclusion ABCA1 expression and cellular cholesterol efflux in macrophages were significantly decreased in OSAHS patients, which closely correlated with the severity of disease. Our findings provide meaningful insights into the mechanism of atherogenesis in OSAHS patients.