Anti-hepatotoxic activities of Hibiscus sabdariffa L. in animal model of streptozotocin diabetes-induced liver damage

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• 作者：David O Adeyemi ; Victor O Ukwenya…
• 关键词：Liver ; Toxicity ; Diabetes mellitus ; Hibiscus sabdariffa ; Flavonoids ; Antioxidant
• 刊名：BMC Complementary and Alternative Medicine
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：14
• 期：1
• 全文大小：2,405 KB
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• 刊物主题：Complementary & Alternative Medicine; Internal Medicine; Chiropractic Medicine;
• 出版者：BioMed Central
• ISSN：1472-6882

文摘

Background Flavonoid-rich aqueous fraction of methanolic extract of Hibiscus sabdariffa calyx was evaluated for its anti-hepatotoxic activities in streptozotocin-induced diabetic Wistar rats. Methods Diabetes Mellitus was induced in Wistar rats by a single i.p injection of 80?mg/kg b.w. streptozotocin (STZ) dissolved in 0.1?M citrate buffer (pH?6.3). Results The ameliorative effects of the extract on STZ-diabetes induced liver damage was evident from the histopathological analysis and the biochemical parameters evaluated in the serum and liver homogenates. Reduced levels of glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) (3.76?±-.38?μM, 0.42?±-.04 U/L, 41.08?±-.04 U/ml, 0.82?±-.04 U/L respectively) in the liver of diabetic rats were restored to a near normal level in the Hibiscus sabdariffa -treated rats (6.87?±-.51?μM, 0.72?±-.06 U/L, 87.92?±-.26 U/ml, 1.37?±-.06 U/L respectively). Elevated levels of aspartate amino transferase (AST), alanine amino transferase (ALT) and alkaline phosphatase (ALP) in the serum of diabetic rats were also restored in Hibiscus sabdariffa -treated rats. Examination of stained liver sections revealed hepatic fibrosis and excessive glycogen deposition in the diabetic rats. These pathological changes were ameliorated in the extract-treated rats. Conclusion The anti-hepatotoxic activity of Hibiscus sabdariffa extract in STZ diabetic rats could be partly related to its antioxidant activity and the presence of flavonnoids.