Aqueous Extracts of Hibiscus sabdariffa Calyces Decrease Hepatitis A Virus and Human Norovirus Surrogate Titers

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• 作者：Snehal S. Joshi ; Lezlee Dice ; Doris H. D’Souza
• 关键词：Hibiscus extracts ; Murine norovirus (MNV ; 1) ; Feline calicivirus (FCV ; F9) ; Hepatitis A virus (HAV) ; Reduction
• 刊名：Food and Environmental Virology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：7
• 期：4
• 页码：366-373
• 全文大小：1,651 KB
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• 作者单位：Snehal S. Joshi (1)
  Lezlee Dice (1)
  Doris H. D’Souza (1)
  
  1. Department of Food Science and Technology, University of Tennessee-Knoxville, Knoxville, TN, 37996, USA
  
• 刊物主题：Virology; Food Science; Chemistry/Food Science, general;
• 出版者：Springer US
• ISSN：1867-0342

文摘

Hibiscus sabdariffa extract is known to have antioxidant, anti-diabetic, and antimicrobial properties. However, their effects against foodborne viruses are currently unknown. The objective of this study was to determine the antiviral effects of aqueous extracts of H. sabdariffa against human norovirus surrogates (feline calicivirus (FCV-F9) and murine norovirus (MNV-1)) and hepatitis A virus (HAV) at 37 °C over 24 h. Individual viruses (~5 log PFU/ml) were incubated with 40 or 100 mg/ml of aqueous hibiscus extract (HE; pH 3.6), protocatechuic acid (PCA; 3 or 6 mg/ml, pH 3.6), ferulic acid (FA; 0.5 or 1 mg/ml; pH 4.0), malic acid (10 mM; pH 3.0), or phosphate buffered saline (pH 7.2 as control) at 37 °C over 24 h. Each treatment was replicated thrice and plaque assayed in duplicate. FCV-F9 titers were reduced to undetectable levels after 15 min with both 40 and 100 mg/ml HE. MNV-1 was reduced by 1.77 ± 0.10 and 1.88 ± 0.12 log PFU/ml after 6 h with 40 and 100 mg/ml HE, respectively, and to undetectable levels after 24 h by both concentrations. HAV was reduced to undetectable levels by both HE concentrations after 24 h. PCA at 3 mg/ml reduced FCV-F9 titers to undetectable levels after 6 h, MNV-1 by 0.53 ± 0.01 log PFU/ml after 6 h, and caused no significant change in HAV titers. FA reduced FCV-F9 to undetectable levels after 3 h and MNV-1 and HAV after 24 h. Transmission electron microscopy showed no conclusive results. The findings suggest that H. sabdariffa extracts have potential to prevent foodborne viral transmission. Keywords Hibiscus extracts Murine norovirus (MNV-1) Feline calicivirus (FCV-F9) Hepatitis A virus (HAV) Reduction