Effect of aqueous extract of Hibiscus sabdariffa on some biochemical parameters in alloxan-induced diabetic rats
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- 作者：Patrick Emeka Aba (1)
Chioma Uchenna Nwaigwe (1)
Flora Ogbonne Okwuagwu (1)
Samuel Chukwuneke Udem (1)
Isaac Uzoma Asuzu (1) - 关键词：Hibiscus sabdariffa ; Diabetes ; Lipid profile ; Liver marker enzymes ; Glycaemia ; Antioxidant
- 刊名：Comparative Clinical Pathology
- 出版年：2014
- 出版时间：November 2014
- 年：2014
- 卷：23
- 期：6
- 页码：1675-1680
- 全文大小：261 KB
- 参考文献：1. Adeneye AA, Olagunja JA (2009) Preliminary hypoglycemic and hypolipidemic activities of aqueous seed extract of / Carica papaya Linn in Wistar rats. Biol Med 1:1-0
2. Aziz OH (2009) Effect of soya beans alone or in combination with insulin or glibenclamide on serum lipid profile in alloxan-induced diabetic rats. Iraqi J Vet Sci 23:17-3
3. Benzie FF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay. Anal Biochem 239:70-6 CrossRef
4. Dafallah AA, Al-Mustafa Z (1996) Investigation of the anti-inflammatory action of / Acacia nilotica and / Hibiscus sabdariffa. Am J Chin Med 24:263-69 CrossRef
5. Dahech I, Belghith KS, Hamden K, Feki A, Belghith H, Mejdoub H (2011) Antidiabetic activity of levan polysaccharide in alloxan-induced diabetic rats. Int J Biol Macromol 49(4):742-46 CrossRef
6. Dalziel JM (1973) The useful plants of West Tropical Africa. Crown Agent for Overseas Government and Administrations, London, pp 129-30
7. Dewanjee S, Bose SK, Sahu R, Mandal SC (2008) Anti-diabetic effect of natural fruits of / Diospyros peregrina in alloxan-induced diabetic rats. Int J Green Pharm 2:95-9 CrossRef
8. Elizabeth HH (2005) Elevated liver function tests in type 2 diabetes mellitus. Clin Diabetes 23:3115-119
9. Hamden K, Allouche N, Damak M, Elfeki A (2009) Hypoglycemic and antioxidant effects of phenolic extracts and purified hydroxytyrosol from olive mill waste in rats. Chem Biol Interact 180(3):421-32 CrossRef
10. Kahn C (2005) The Merck veterinary manual. Merck, Whitehouse Station, NJ
11. Klein B, Read PA, Babson AL (1960) Rapid method for the quantitative determination of serum alkaline phosphatase. Clin Chem 6:269-75
12. Mahdi AA, Chandra A, Singh RK, Mishra LC, Ahmad S (2003) Effect of herbal hypoglycemic agents on oxidative stress and antioxidant status in diabetic rats. Indian J Clin Biochem 18(2):8-5 CrossRef
13. Mozaffari-khosravi H, Jalali-khanabadi BA, Afkhami-Ardekani M, Fetehi F (2009) Effect of sour tea ( / Hibiscus sabdariffa) on lipid profile and lipoproteins in patient with type II diabetes. J Altern Complement Med 15(8):899-03 CrossRef
14. Odigie IP, Ettarh RR, Adigun SA (2003) Chronic administration of aqueous extract of / Hibiscus sabdariffa attenuates hypertension and reverses cardiac hypertrophy in 2K-1 C hypertensive rats. J Ethnopharmacol 86:181-85 CrossRef
15. Parasuraman S, Raveendran R, Kesavan R (2010) Blood sample collection in small laboratory animals. J Pharmacol Pharmacother 1(2):87-3 CrossRef
16. Reitman S, Frankel S (1957) Colorimetric method for determination of serum transaminases. Am J Clin Pathol 28:56-1
17. Sakurai K, Katoh M, Someno K, Fujimoto Y (2001) Apoptosis and mitochondrial damage in INS-1 cells treated with alloxan. Biol Pharm Bull 24(8):876-82 CrossRef
18. Sepici-Dincel A, Acikgoz S, Cevik C, Sengelen M, Yesilada E (2007) Effects of in vivo antioxidant enzyme activities of myrtle oil in normoglycaemic and alloxan diabetic rabbits. J Ethnopharmacol 110(3):498-03 CrossRef
19. Swantson-Flatt SK, Day C, Bailey CJ, Flatt PR (1990) Traditional treatments for diabetes: studies in normal and streptozotocin diabetic mice. Diabetologia 33:462-64 CrossRef
20. Szkudelski T (2001) The mechanism of alloxan and streptozotocin action in B-cells of the rats pancreas. Physiol Res 50:536-46
21. Turner CD (1961) General endocrinology. W.B. Saunder, London, pp 182-90
22. Twari AK, Rao J - 作者单位：Patrick Emeka Aba (1)
Chioma Uchenna Nwaigwe (1)
Flora Ogbonne Okwuagwu (1)
Samuel Chukwuneke Udem (1)
Isaac Uzoma Asuzu (1)
1. Department of Veterinary Physiology and Pharmacology, University of Nigeria, Nsukka, Enugu State, Nigeria - ISSN：1618-565X
文摘
This study investigated some biochemical effects associated with subchronic administration of the aqueous extract of Hibiscus sabdariffa to alloxan-induced diabetic rats. Twenty male albino Wistar rats were assigned to four groups of five rats per group. Diabetes was induced in rats in groups 1, 2 and 3 by a single intraperitoneal injection of alloxan monohydrate (150?mg/kg) while rats in group 4 served as positive control. Upon establishment of diabetes, rats in group 1 were treated with 30?mg/ml of H. sabdariffa extract ad libitum while those in group 3 were treated with glibenclamide (2?mg/kg), a known anti-diabetic drug. Rats in groups 2 and 4 were given clean water. All treatment lasted for 21?days. Blood glucose levels of the rats were assessed on days 0, 7, 14 and 21 while blood for clinical chemistry analyses (alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total cholesterol, triglycerides, high density lipoprotein) were collected on days 0 and 21. Low density lipoprotein (LDL) was calculated using Friedewald formula and very low density lipoprotein was gotten by dividing triglyceride by 5. In vitro antioxidant assay was carried out using ferric reducing antioxidant power model. Results showed that the blood glucose level of the extract-treated rats decreased significantly (p--.05) from 296.00?±-6.11 on day 0 to 30.87?±-1.39 on day 21. This was comparable with reduction achieved by glibenclamide-treated group. The total cholesterol and LDL levels of the extract-treated rats were significantly (p--.05) lower than that of the diabetic-untreated controls. There was no significant difference (p--.05) in the mean activities of serum AST, ALT and ALP when compared across the groups. The results of in vitro antioxidant test revealed that the antioxidant activity of H. sabdariffa extract was concentration-dependent with the maximum antioxidant activity of 87?% at 400?mg/ml. The aqueous extract of H. sabdariffa exhibited hypoglycaemic, hypolipidaemic and antioxidant effects on alloxan-induced diabetic rats.