Micropropagation for mass multiplication and enriched production of ascorbic acid in tissue culture foliage of roselle (Hibiscus sabdariffa L.)

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• 作者：Sandopu Sravan Kumar ; Prabhakaran Manoj…
• 关键词：Micropropagation ; Nodal explants ; Multiple shoots ; In vitro rooting ; Silver nitrate ; Triacontanol
• 刊名：In Vitro Cellular & Developmental Biology - Plant
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：52
• 期：4
• 页码：427-436
• 全文大小：972 KB
• 刊物主题：Plant Sciences; Cell Biology; Developmental Biology; Plant Breeding/Biotechnology; Plant Genetics & Genomics;
• 出版者：Springer US
• ISSN：1475-2689
• 卷排序：52

文摘

An efficient protocol was developed for the micropropagation of Hibiscus sabdariffa through nodal explants (auxillary bud cultures) of in vitro seedlings. Nodal explants were able to induce multiple shoots on Murashige and Skoog (MS) basal medium supplemented with 2.0 mg L−1 6-benzylaminopurine, 0.5 mg L−1 indole-3-acetic acid, and 10 μM silver nitrate (AgNO₃; 7–8 shoots/explant) or 20 μg L−1 triacontanol (TRIA; 4–5 shoots/explant). Medium devoid of AgNO₃ that was supplemented with 0.1–0.5 mg L−1 gibberellic acid (GA₃) induced the proliferation of up to two shoots/explant, but supplementation with 1–5 mg L−1 GA₃ induced a single shoot. The highest frequency of shoot elongation and rooting was obtained on 0.5-strength MS medium supplemented with 0.1 or 0.5 mg L−1 GA₃, and on this medium, further proliferation of shoots was also evident. Micropropagated plants were hardened in the greenhouse and successfully established in soil. Ascorbic acid level in leaves of micropropagated plants from AgNO₃- and TRIA-supplemented media was highest (2.24 ± 0.14 and 2.18 ± 0.13 mg g−1 fresh weight, respectively), which was 2.8-fold more than normal ex vitro leaves of the same age (0.79 ± 0.03 mg g−1 fresh weight).