Efficacy of Phage and Ciprofloxacin Co-therapy on the Formation and Eradication of Pseudomonas aeruginosa Biofilms

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• 作者：Sadhana Singh Sagar ; Rajesh Kumar…
• 关键词：Bacteriophage ; TEM ; Ciprofloxacin ; Biofilm and Pseudomonas aeruginosa
• 刊名：Arabian Journal for Science and Engineering
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：42
• 期：1
• 页码：95-103
• 全文大小：
• 刊物类别：Engineering
• 刊物主题：Engineering, general; Science, Humanities and Social Sciences, multidisciplinary;
• 出版者：Springer Berlin Heidelberg
• ISSN：2191-4281
• 卷排序：42

文摘

Pseudomonas aeruginosa, a gram negative, multiple drug-resistant (MDR) biofilm forming organism associated with nosocomial infections, is the causative agent of several life-threatening diseases. Emergence of multiple drug resistance against current generation antibiotics in such pathogens is a serious problem for medical practitioners. An alternative means to control the MDR pathogens is combination therapy of antibiotic with suitable biological control measures such as lytic bacteriophages. In this study, we report the isolation and characterization of P. aeruginosa specific phage vB_PaeM_P6 which is used in combination with sub-minimal inhibitory concentrations (MIC) of ciprofloxacin for the control of P. aeruginosa biofilm. Synergistic activity of phage 0.05 multiplicity of infection and sub-MIC ciprofloxacin (0.75 \({\mu}\)g/ml) treatment is reported for the inhibition of biofilm forming as well as preformed biofilms of five clinical strains of P. aeruginosa.