Skin donors and human skin allografts: evaluation of an 11-year practice and discard in a referral tissue bank

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• 作者：Sonia Gaucher ; Zena Khaznadar ; Jean-Claude Gourevitch…
• 关键词：Tissue banking ; Skin donors ; Skin allografts ; Discards ; Microbial contamination
• 刊名：Cell and Tissue Banking
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：17
• 期：1
• 页码：11-19
• 全文大小：378 KB
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• 作者单位：Sonia Gaucher (1) (2)
  Zena Khaznadar (3)
  Jean-Claude Gourevitch (3)
  Mohamed Jarraya (3)
  
  1. Faculté de Médecine, Université Paris Descartes, Paris, France
  2. Service de Chirurgie Générale, Plastique et Ambulatoire, APHP, Hôpitaux Universitaires Paris Centre, Hôpital Cochin, Paris, France
  3. Banque des Tissus Humains, APHP, Hôpital Saint-Louis, 1 Avenue Claude Vellefaux, 75 010, Paris, France
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Biomedicine
  Transplant Surgery
  
• 出版者：Springer Netherlands
• ISSN：1573-6814

文摘

The Saint Louis hospital tissue bank provides skin allografts to pediatric and adult burn units in the Paris area. The aim of this study was to analyze our activity during the last 11 years focusing on the reasons for skin discard. Skin is procured solely from the back of the body, which is divided into 10 zones that are harvested and processed separately. This retrospective study included all skin donors harvested between June 2002 and June 2013, representing a total of 336 donors and 2770 zones. The donors were multiorgan heart-beating donors in 91 % of cases (n = 307). The main reason for discarding harvested skin was microbial contamination, detected in 99 donors (29 %). Most contaminants were of low pathogenicity. Other reasons for discard included positive serologic tests for 2 donors [17 zones (0.61 %)], unsuitable physical skin characteristics for 3 zones (0.11 %), the donor’s medical history for 53 zones (1.91 %), and technical issues with processing or distribution for 61 zones (2.2 %). In our experience, microbial contamination continues to be the main reason for discarding potential skin allografts. However, discards are limited by separate harvesting and processing of multiple zones in each donor.