Assessing similarity analysis of chromatographic fingerprints of Cyclopia subternata extracts as potential screening tool for in vitro glucose utilisation

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• 作者：Alexandra E. Schulze ; Dalene De Beer…
• 关键词：Honeybush ; Chemometric analysis ; Glucose uptake ; Mangiferin ; Isomangiferin ; Scolymoside
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：408
• 期：2
• 页码：639-649
• 全文大小：784 KB
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• 作者单位：Alexandra E. Schulze (1) (2)
  Dalene De Beer (3)
  Sithandiwe E. Mazibuko (4)
  Christo J. F. Muller (4)
  Candice Roux (4)
  Elize L. Willenburg (3)
  Nyemb Nyunaï (4) (5)
  Johan Louw (4)
  Marena Manley (1)
  Elizabeth Joubert (1) (3)
  
  1. Department of Food Science, Stellenbosch University, Private Bag X1, Matieland, Stellenbosch, 7602, South Africa
  2. Department of Viticulture and Oenology, Stellenbosch University, Private Bag X1, Matieland, Stellenbosch, 7602, South Africa
  3. Post-Harvest and Wine Technology Division, Agricultural Research Council (ARC), Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch, 7599, South Africa
  4. Diabetes Discovery Platform, South African Medical Research Council, P.O. Box 19070, Tygerberg, 7505, South Africa
  5. Medical Research Center, Institute of Medical Research and Medicinal Plants Studies, P.O. Box 3805, Yaoundé, Cameroon
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Food Science
  Inorganic Chemistry
  Physical Chemistry
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1618-2650

文摘

Similarity analysis of the phenolic fingerprints of a large number of aqueous extracts of Cyclopia subternata, obtained by high-performance liquid chromatography (HPLC), was evaluated as a potential tool to screen extracts for relative bioactivity. The assessment was based on the (dis)similarity of their fingerprints to that of a reference active extract of C. subternata, proven to enhance glucose uptake in vitro and in vivo. In vitro testing of extracts, selected as being most similar (n = 5; r ≥ 0.962) and most dissimilar (n = 5; r ≤ 0.688) to the reference active extract, showed that no clear pattern in terms of relative glucose uptake efficacy in C2C12 myocytes emerged, irrespective of the dose. Some of the most dissimilar extracts had higher glucose-lowering activity than the reference active extract. Principal component analysis revealed the major compounds responsible for the most variation within the chromatographic fingerprints, as mangiferin, isomangiferin, iriflophenone-3-C-β-d-glucoside-4-O-β-d-glucoside, iriflophenone-3-C-β-d-glucoside, scolymoside, and phloretin-3′,5′-di-C-β-d-glucoside. Quantitative analysis of the selected extracts showed that the most dissimilar extracts contained the highest mangiferin and isomangiferin levels, whilst the most similar extracts had the highest scolymoside content. These compounds demonstrated similar glucose uptake efficacy in C2C12 myocytes. It can be concluded that (dis)similarity of chromatographic fingerprints of extracts of unknown activity to that of a proven bioactive extract does not necessarily translate to lower or higher bioactivity.