Human Serum Potentiates the Expression of Genes Associated with Antifungal Drug Resistance in C. albicans Biofilms on Central Venous Catheters

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• 作者：L. P. Samaranayake (1) (2)
  S. Anil (3)
  M. Hashem (4)
  S. Vellappally (4)
  B. P. K. Cheung (5)
  
  1. School of Dentistry ; Level 7 ; UQ Oral Health Centre ; University of Queensland ; Herston Road ; Brisbane ; QLD ; 4006 ; Australia
  2. College of Applied Medical Sciences ; King Saud University ; Riyadh ; Saudi Arabia
  3. Department of Periodontics and Community Dentistry ; College of Dentistry ; King Saud University ; Riyadh ; Saudi Arabia
  4. Dental Biomaterials Research Chair ; Dental Health Department ; College of Applied Medical Sciences ; King Saud University ; Riyadh ; 11433 ; Saudi Arabia
  5. Oral Biosciences ; Faculty of Dentistry ; University of Hong Kong ; 34 ; Hospital Road ; Hong Kong ; China
  
• 关键词：Serum ; Drug resistance genes ; C. albicans biofilms ; Central venous catheters
• 刊名：Mycopathologia
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：179
• 期：3-4
• 页码：195-204
• 全文大小：732 KB
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• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Medical Microbiology
  Plant Sciences
  Microbial Ecology
  
• 出版者：Springer Netherlands
• ISSN：1573-0832

文摘

Candida albicans is a major agent of fungaemias and frequently causes systemic disease through seeded, blood stream dissemination. These infections, particularly common in hospitalized patients with central venous catheters (CVCs), appear to persevere due to biofilm reservoirs of the yeast that tend to develop on the device. Although it is known that candidal biofilms are intrinsically resistant to antifungals compared with their planktonic counterparts, there is a paucity of data on the expression of antifungal drug resistance genes (DRGs) in candidal biofilms in CVC reservoirs. Furthermore, notwithstanding the fact that CVCs are constantly bathed in human serum, there are no studies on the effect of the latter on the DRG expression in candidal biofilms. Hence, we developed in vitro biofilms of three different C. albicans strains on silicone CVC discs immersed in human serum and evaluated the temporal expression of nine antifungal DRGs. In an attempt to evaluate the effect of hyphal elements on DRG expression, we incorporated a hyphal mutant (HM) and its wild-type (WT) counterpart, as well as a fresh clinical isolate in the studies. Human serum significantly up-regulated DRG transcripts in Candida biofilms on CVCs, at different stages of biofilm growth, while the WT strain over-expressed more DRGs than the HM strain. Here, we report, for the first time, that both human serum and the hyphal elements of the yeast have a profound modulatory effect on DRG expression in C. albicans biofilms on CVCs.