Simultaneous fluorescence detection of mercury (II) and silver ions based on rhodamine B isothiocyanate and 5-carboxyfluorescein-ssDNA modified probe

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• 作者：Yinran Chen ; Kang Mao ; Xiaodong Zhou…
• 关键词：Key wordsfluorescence sensor ; simultaneous detection ; metal ions
• 刊名：Wuhan University Journal of Natural Sciences
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：21
• 期：6
• 页码：499-504
• 全文大小：418 KB
• 刊物类别：Mathematics and Statistics
• 刊物主题：Mathematics
  Computer Science, general
  Physics
  Life Sciences
  Chinese Library of Science
  
• 出版者：Wuhan University, co-published with Springer
• ISSN：1993-4998
• 卷排序：21

文摘

A new fluorescence sensor is developed for simultaneous detection of Hg2+ and Ag+. During the detection process, Hg2+ forms complexes with the fluorescent dye rhodamine B isothiocyanate (RBITC) modified onto the surface of gold nanoparticles(AuNPs), resulting in RBITC’s displacement from the surface of AuNPs and the recovery of fluorescence. Meanwhile, Ag+ forms “C-Ag+-C” complex with C-rich 5-carboxyfluorescein (FAM)-ssDNA modified onto the surface of AuNPs, which keeps the fluorescent dye FAM close to the AuNPs and results in quench of fluorescence. The experimental results show a wide linear range and a good sensitivity. The limit of detection is 1.06 nmol/L for Ag+ and 0.48 nmol/L for Hg2+. This detection method is not only easy to operate but also efficient.