Evaluation of molecular genetic diversity of wild apple Malus sieversii populations from Zailiysky Alatau by microsatellite markers

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• 作者：M. E. Omasheva ; S. V. Chekalin ; N. N. Galiakparov
• 刊名：Russian Journal of Genetics
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：51
• 期：7
• 页码：647-652
• 全文大小：1,789 KB
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• 作者单位：M. E. Omasheva (1)
  S. V. Chekalin (2)
  N. N. Galiakparov (1)
  
  1. Institute of Plant Biology and Biotechnology, Almaty, 05040, Republic of Kazakhstan
  2. Institute of Botany and Phytointroduction, Almaty, 05040, Republic of Kazakhstan
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Human Genetics
  Animal Genetics and Genomics
  Microbial Genetics and Genomics
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-3369

文摘

The territory of Kazakhstan is part of the distribution range of Malus sieversii, which is one of the ancestors of cultivated apple tree varieties. The collected samples of Sievers apple leaves from five populations growing in the Zailiysky Alatau region served as a source not only for the creation of a bank of genomic DNA but also for determination of the wild apple genetic polymorphism. The seven microsatellite markers used in this study revealed 86 alleles with different frequencies, as well as the characteristic pools of rare alleles for each of the populations. Molecular genetic analysis showed a high level of genetic diversity (H o = 0.704, PIC = 0.752, I = 1.617). Moreover, interpopulation variability accounted only for 7.5% of total variability, confirming the genetic closeness of the populations examined. Based on phylogenetic analysis, it was demonstrated that the Bel’bulak and Almaty Reserve populations were closest to each other, while the most distant were the Ketmen and Great Almaty gorge populations, which suggests the dependence of genetic distance on the geographical.