Total antioxidant capacity of honeybee haemolymph in relation to age and exposure to pesticide, and comparison to antioxidant capacity of seminal plasma

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• 作者：Mariola Słowińska ; Joanna Nynca ; Jerzy Wilde ; Beata Bąk ; Maciej Siuda…
• 关键词：Apis mellifera ; total antioxidant capacity ; haemolymph ; ageing ; pesticide
• 刊名：Apidologie
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：47
• 期：2
• 页码：227-236
• 全文大小：708 KB
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• 作者单位：Mariola Słowińska (1) <br> Joanna Nynca (1) <br> Jerzy Wilde (2) <br> Beata Bąk (2) <br> Maciej Siuda (2) <br> Andrzej Ciereszko (1) <br><br>1. Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences in Olsztyn, Tuwima 10, 10-748, Olsztyn, Poland <br> 2. Department of Apiculture, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Słoneczna 48, 10-957, Olsztyn, Poland <br>
• 刊物主题：Entomology; Life Sciences, general;
• 出版者：Springer Paris
• ISSN：1297-9678

文摘

Oxidative stress is defined as a disturbance in the balance between the production of reactive oxygen species and antioxidant defences. We measured total antioxidant capacity (TAC) in honeybee haemolymph and seminal plasma and analysed TAC of haemolymph in relation to age and exposure to pesticide. TAC of haemolymph increased with age of bees (1.18 vs 1.97 mM of (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) for 1- and 30-day-old bees, respectively, P ≤ 0.05). Exposure to imidacloprid (IMD) affected TAC of haemolymph of 1-day-old but not 30-day-old honeybees. TAC in haemolymph of 1-day-old bees was lower in treatments with the addition of 5 and 200 ppb IMD (1.57–1.46 mM of Trolox in treated bees compared with 2.37 mM of Trolox in controls; P ≤ 0.05). In conclusion, antioxidant protection of honeybees seems to be related to age and may be disturbed by exposure to IMD. Older bees with higher antioxidant protection seem to be less susceptible to IMD toxicity. The toxic effect of pesticide seems to be particularly dangerous in early life stages of honeybees.