Faecal carriage of carbapenemase-producing Gram-negative bacilli in hospital settings in southern France

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• 作者：A. Pantel ; H. Marchandin ; M.-F. Prère…
• 刊名：European Journal of Clinical Microbiology & Infectious Diseases
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：34
• 期：5
• 页码：899-904
• 全文大小：479 KB
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• 作者单位：A. Pantel (1) (2)
  H. Marchandin (3)
  M.-F. Prère (4)
  A. Boutet-Dubois (2) (5)
  N. Brieu-Roche (6)
  A. Gaschet (7)
  A. Davin-Regli (8)
  A. Sotto (2) (9)
  J.-P. Lavigne (1) (2)
  
  1. Service de Microbiologie, CHU Carémeau, Place du Professeur Robert Debré, N?mes, France
  2. Institut National de la Santé et de la Recherche Médicale, U1047, Université Montpellier 1, N?mes, France
  3. Laboratoire de Bactériologie, CHU Arnaud de Villeneuve, Montpellier, France
  4. Laboratoire de Bactériologie-Hygiène, CHU Purpan, Toulouse, France
  5. Laboratoire de Biologie Polyvalente, CH Alès-Cévennes, Alès, France
  6. Laboratoire de Biologie Polyvalente, CH du Pays d’Aix, Aix-en-Provence, France
  7. Laboratoire de Biologie Polyvalente, CH Saint-Jean, Perpignan, France
  8. Laboratoire de Biologie Polyvalente, CH Edmond Garcin, Aubagne, France
  9. Service des Maladies Infectieuses et Tropicales, CHU Carémeau, N?mes, France
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Medical Microbiology
  Internal Medicine
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1435-4373

文摘

The emergence of carbapenemase-producing Gram-negative bacilli is a worldwide problem. To date, no study has evaluated the prevalence of faecal carriage of carbapenemase-producing and carbapenem-resistant Gram-negative bacilli (CR GNB) in France. From 1 February to 30 April 2012, we conducted a prospective, multicentre study in three University Hospitals and four General Hospitals in the south of France. The carriage of carbapenemase-producing Enterobacteriaceae (CPE) and other CR GNB was screened by both cultivation on chromID? CARBA and chromID? OXA-48 media (bioMérieux) and molecular tools [multiplex polymerase chain reaction (PCR) and NucliSENS EasyQ? KPC (bioMérieux)]. The genetic relationship between isolates was assessed by rep-PCR (DiversiLab, bioMérieux) or multilocus sequence typing (MLST). The prevalences of CR GNB and carbapenemase-producing bacteria were 2.4?% (27/1,135) and 0.4?% (n--), respectively. Two strains corresponded to OXA-23-producing Acinetobacter baumannii and belonged to the widespread sequence type (ST) 2/international clone II, whereas one strain was an ST15 OXA-48-producing Klebsiella pneumoniae. Two OXA-48-producers were detected exclusively by PCR. This first French study revealed the very low dissemination of carbapenemase-producing bacteria in patients attending hospitals in southern France during a non-outbreak situation. However, the increasing description of epidemic cases in this area must reinforce the use of hygiene procedures to prevent diffusion of these multidrug-resistant microorganisms.