Effects of whole cigarette smoke on human beta defensins expression and secretion by oral mucosal epithelial cells

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• 作者：Wen-mei Wang (1)
  Pei Ye (1)
  Ya-jie Qian (1)
  Ya-fan Gao (1)
  Jing-jing Li (1)
  Fang-fang Sun (2) (3)
  Wei-yun Zhang (3)
  Xiang Wang (1) (3)
  
  1. Department of Oral Medicine ; Institute and Hospital of Stomatology ; Nanjing University Medical School ; 30 Zhongyang Road ; Nanjing ; 210008 ; China
  2. Department of Prosthodontics ; Institute and Hospital of Stomatology ; Nanjing University Medical School ; Nanjing ; 21008 ; China
  3. Immunology and Reproduction Biology Laboratory ; Medical School ; Nanjing University ; 22 Hankou Road ; Nanjing ; 210093 ; China
  
• 关键词：Whole cigarette smoke ; Human 尾 defensin ; Oral mucosa
• 刊名：Tobacco Induced Diseases
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：13
• 期：1
• 全文大小：3,029 KB
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• 刊物主题：Public Health; Behavioural Sciences; Internal Medicine; Health Psychology;
• 出版者：BioMed Central
• ISSN：1617-9625

文摘

Background Cigarette smoke a recognized risk factor for many systemic diseases and also oral diseases. Human beta defensins (HBDs), a group of important antimicrobial peptides expressed by the epithelium, are crucial for local defense and tissue homeostasis of oral cavity. The aim of this study was to evaluate potential effects of whole cigarette smoke (WCS) exposure on the expression and secretion of HBDs by oral mucosal epithelial cells. Methods Immortalized human oral mucosal epithelial (Leuk-1) cells were exposed to WCS for various time periods. HBD-1, -2 and -3 expression and subcellular localization were detected by real time qPCR, immunofluorescence assay and confocal microscopy. According to the relative fluorescent intensity, the expression levels of HBD-1, -2 and -3 were evaluated by digital image analysis system. The alteration of HBD-1, -2 and -3 secretion levels was measured by the Enzyme-Linked Immunosorbent Assay. Results WCS exposure remarkably attenuated HBD-1 expression and secretion while clearly enhanced HBD-2, -3 expression levels and HBD-2 secretion by Leuk-l cells. It appeared that there was no significant effect of WCS exposure on HBD-3 secretion. Conclusions WCS exposure could modulate expression and secretion of HBDs by oral mucosal epithelial cells, establishing a link between cigarette smoke and abnormal levels of antimicrobial peptides. The present results may give a new perspective to investigate smoking-related local defense suppression and oral disease occurrence.