The effects of multifunctional MiR-122-loaded graphene-gold composites on drug-resistant liver cancer

详细信息    查看全文

• 作者：Yi Yuan (3)
  Yaqin Zhang (4)
  Bin Liu (5)
  Heming Wu (3)
  Yanjun Kang (7)
  Ming Li (3)
  Xin Zeng (2) (6)
  Nongyue He (2)
  Gen Zhang (1) (2)
  
  3. Institute of Stomatology ; Nanjing Medical University ; Nanjing ; 210029 ; China
  4. Department of Biochemistry and Molecular Biology ; Nanjing Medical University ; Nanjing ; 210029 ; China
  5. Department of Biomedical Engineering ; Nanjing Medical University ; Nanjing ; 210029 ; China
  7. Jiangnan University Medical School ; Wuxi ; Jiangsu ; 214122 ; China
  2. The State Key Laboratory of Bioelectronics ; Department of Biological Science and Medical Engineering ; Southeast University ; Nanjing ; 210096 ; China
  6. Maternal and Child Health Institute ; Nanjing Maternity and Child Health Care Hospital ; Nanjing ; 210029 ; China
  1. Department of Cell Biology ; Nanjing Medical University ; Nanjing ; 210029 ; China
  
• 关键词：Graphene ; Gold nanoparticles ; Cell apoptosis ; Control release ; Target
• 刊名：Journal of Nanobiotechnology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：13
• 期：1
• 全文大小：4,106 KB
• 参考文献：1. Pathak, A, Vyas, SP, Gupta, KC (2008) Nano-vectors for efficient liver specific gene transfer. Int J Nanomedicine 3: pp. 31-49 CrossRef
  2. Ruan, J, Song, H, Li, C, Bao, C, Fu, H, Wang, K (2012) DiR-labeled Embryonic Stem Cells for Targeted Imaging of in vivo Gastric Cancer Cells. Theranostics 2: pp. 618-28 CrossRef
  3. Basu, S, Bhattacharyya, SN (2014) Insulin-like growth factor-1 prevents miR-122 production in neighbouring cells to curtail its intercellular transfer to ensure proliferation of human hepatoma cells. Nucleic Acids Res 42: pp. 7170-85 CrossRef
  4. Filipowicz, W, Grosshans, H (2011) The liver-specific microRNA miR-122: biology and therapeutic potential. Prog Drug Res 67: pp. 221-38
  5. Hou, W, Bukong, TN, Kodys, K, Szabo, G (2013) Alcohol facilitates HCV RNA replication via up-regulation of miR-122 expression and inhibition of cyclin G1 in human hepatoma cells. Alcohol Clin Exp Res 37: pp. 599-608 CrossRef
  6. Li, W, Abu Samra, D, Merzaban, J, Khashab, NM (2013) P-glycoprotein targeted nanoscale drug carriers. J Nanosci Nanotechnol 13: pp. 1399-402 CrossRef
  7. Vlerken, LE, Amiji, MM (2006) Multi-functional polymeric nanoparticles for tumour-targeted drug delivery. Expert Opin Drug Deliv 3: pp. 205-16 CrossRef
  8. Chiappetta, DA, Sosnik, A (2007) Poly(ethylene oxide)-poly(propylene oxide) block copolymer micelles as drug delivery agents: improved hydrosolubility, stability and bioavailability of drugs. Eur J Pharm Biopharm 66: pp. 303-17 CrossRef
  9. Hayama, A, Yamamoto, T, Yokoyama, M, Kawano, K, Hattori, Y, Maitani, Y (2008) Polymeric micelles modified by folate-PEG-lipid for targeted drug delivery to cancer cells in vitro. J Nanosci Nanotechnol 8: pp. 3085-90 CrossRef
  10. Sahu, A, Choi, WI, Lee, JH, Tae, G (2013) Graphene oxide mediated delivery of methylene blue for combined photodynamic and photothermal therapy. Biomaterials 34: pp. 6239-48 CrossRef
  11. Yoshida, GJ, Fuchimoto, Y, Osumi, T, Shimada, H, Hosaka, S, Morioka, H (2012) Li-Fraumeni syndrome with simultaneous osteosarcoma and liver cancer: increased expression of a CD44 variant isoform after chemotherapy. BMC Cancer 12: pp. 444 CrossRef
  12. Stark, GR (1986) Cancer chemotherapy. Progress in understanding multidrug resistance. Nature 324: pp. 407-8 CrossRef
  13. Solarska, K, Gajewska, A, Bartosz, G, Mitura, K (2012) Induction of apoptosis in human endothelial cells by nanodiamond particles. J Nanosci Nanotechnol 12: pp. 5117-21 CrossRef
  14. Long, Q, Xiel, Y, Huang, Y, Wu, Q, Zhang, H, Xiong, S (2013) Induction of apoptosis and inhibition of angiogenesis by PEGylated liposomal quercetin in both cisplatin-sensitive and cisplatin-resistant ovarian cancers. J Biomed Nanotechnol 9: pp. 965-75 CrossRef
  15. Gui, C, Cui, DX (2012) Functionalized gold nanorods for tumor imaging and targeted therapy. Cancer Biol Med 9: pp. 221-33
  16. Khlebtsov, N, Bogatyrev, V, Dykman, L, Khlebtsov, B, Staroverov, S, Shirokov, A (2013) Analytical and theranostic applications of gold nanoparticles and multifunctional nanocomposites. Theranostics 3: pp. 167-80 CrossRef
  17. Bellamy, WT (1996) P-glycoproteins and multidrug resistance. Annu Rev Pharmacol Toxicol 36: pp. 161-83 CrossRef
  18. Hou, Z, Zhan, C, Jiang, Q, Hu, Q, Li, L, Chang, D (2011) Both FA- and mPEG-conjugated chitosan nanoparticles for targeted cellular uptake and enhanced tumor tissue distribution. Nanoscale Res Lett 6: pp. 563 CrossRef
  19. Zhao, R, Hanscom, M, Chattopadhyay, S, Goldman, ID (2004) Selective preservation of pemetrexed pharmacological activity in HeLa cells lacking the reduced folate carrier: association with the presence of a secondary transport pathway. Cancer Res 64: pp. 3313-9 CrossRef
  20. McGuire, JJ, Haile, WH, Yeh, CC (2006) 5-amino-4-imidazolecarboxamide riboside potentiates both transport of reduced folates and antifolates by the human reduced folate carrier and their subsequent metabolism. Cancer Res 66: pp. 3836-44 CrossRef
  21. Lin, CJ, Gong, HY, Tseng, HC, Wang, WL, Wu, JL (2008) miR-122 targets an anti-apoptotic gene, Bcl-w, in human hepatocellular carcinoma cell lines. Biochem Biophys Res Commun 375: pp. 315-20 CrossRef
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Biotechnology
  Nanotechnology
  
• 出版者：BioMed Central
• ISSN：1477-3155

文摘

Background Nano drugs have attracted increased attention due to their unique mode of action that offers tumor-inhibiting effects. Therefore, we have previously explored functionalized and drug-loaded graphene-gold nanocomposites that induced cancer cell apoptosis. Results In the present study, we developed a combination of monoclonal P-glycoprotein (P-gp) antibodies, folic acid (FA) and miR-122-loaded gold nanoparticles on graphene nanocomposites (GGMPN), which promoted drug-resistant HepG2 cell apoptosis with drug targeting and controlled release properties. We also investigated related apoptosis proteins and apoptosis signal pathways by GGMPN treatment in vitro and in vivo. Moreover, we further demonstrated the inhibition of tumor growth and the apoptosis-inducing effect by means of GGMPN with a semiconductor laser in a xenograft tumor model. Conclusion In conclusion, our results collectively suggested that GGMPN could serve as a novel therapeutic approach to control tumor cell apoptosis and growth.