Flow of essential elements in subcellular fractions during oxidative stress

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• 作者：Larissa Lago ; Emilene A. Nunes ; Aryane A. Vigato ; Vanessa C. O. Souza…
• 关键词：Metal quantification ; Metal flow ; Oxidative stress ; Neuroscience
• 刊名：BioMetals
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：30
• 期：1
• 页码：83-96
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biochemistry, general; Pharmacology/Toxicology; Microbiology; Cell Biology; Plant Physiology; Medicine/Public Health, general;
• 出版者：Springer Netherlands
• ISSN：1572-8773
• 卷排序：30

文摘

Essential trace elements are commonly found in altered concentrations in the brains of patients with neurodegenerative diseases. Many studies in trace metal determination and quantification are conducted in tissue, cell culture or whole brain. In the present investigation, we determined by ICP-MS Fe, Cu, Zn, Ca, Se, Co, Cr, Mg, and Mn in organelles (mitochondria, nuclei) and whole motor neuron cell cultured in vitro. We performed experiments using two ways to access oxidative stress: cell treatments with H₂O₂ or Aβ-42 peptide in its oligomeric form. Both treatments caused accumulation of markers of oxidative stress, such as oxidized proteins and lipids, and alteration in DNA. Regarding trace elements, cells treated with H₂O₂ showed higher levels of Zn and lower levels of Ca in nuclei when compared to control cells with no oxidative treatments. On the other hand, cells treated with Aβ-42 peptide in its oligomeric form showed higher levels of Mg, Ca, Fe and Zn in nuclei when compared to control cells. These differences showed that metal flux in cell organelles during an intrinsic external oxidative condition (H₂O₂ treatment) are different from an intrinsic external neurodegenerative treatment.