Changes in the NMR Metabolic Profile of Live Human Neuron-Like SH-SY5Y Cells Exposed to Interferon-α2

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• 作者：Righi Valeria ; Schenetti Luisa ; Mucci Adele…
• 关键词：IFN ; α ; SH ; SY5Y cells ; Metabolomics ; HR ; MAS NMR ; Osmolytes ; Neurotoxicity
• 刊名：Journal of Neuroimmune Pharmacology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：11
• 期：1
• 页码：142-152
• 全文大小：1,004 KB
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• 作者单位：Righi Valeria (1)
  Schenetti Luisa (2)
  Mucci Adele (3)
  Benatti Stefania (2)
  Tascedda Fabio (2)
  Brunello Nicoletta (2)
  Pariante M Carmine (4)
  Alboni Silvia (2)
  
  1. Department for Life Quality Studies, University of Bologna, Rimini, Italy
  2. Department of Life Sciences, University of Modena and Reggio Emilia, via G. Campi, 287, 41125, Modena, Italy
  3. Department of Chemical and Geological Sciences, University of Modena and Reggio Emilia, Modena, Italy
  4. Stress, Psychiatry and Immunology Department of Psychological Medicine Institute of Psychiatry, Kings College London, London, UK
  
• 刊物主题：Neurosciences; Immunology; Pharmacology/Toxicology; Virology; Cell Biology;
• 出版者：Springer US
• ISSN：1557-1904

文摘

Interferon (IFN)-α2 is an extensively therapeutically used pro-inflammatory cytokine. Though its efficacy in controlling viral replication and tumor cells proliferation, administration of IFN-α2 is often associated with the development of central side effects. Magnetic resonance spectroscopy studies have demonstrated that IFN-α2 administration affects brain metabolism, however the exact nature of this effect is not completely known. We hypothesized that IFN-α2 can affect metabolic activity of human neuron-like SH-SY5Y cells which possess many characteristics of neurons and represent one of the most used models for studying mechanisms involved in neurotoxicity or neuroprotection. To test our hypothesis we have characterized the metabolic signature of live SH-SY5Y, and their conditioned media, after 24 and 72 h of exposure to vehicle or IFN-α2 (100 ng/ml) by using High Resolution-Magic Angle Spinning (HR-MAS) Nuclear Magnetic Resonance (NMR) spectroscopy. Our results revealed that 1) the use of HR-MAS NMR is ideally suitable for the characterization of the metabolic profile of live cells and their conditioned media without extraction procedures; and 2) a 72 h exposure to IFN-α2 increases the level of metabolites involved in maintaining energetic (including creatine and lactate) and osmotic (such as myo-inositol, scyllo-inositol, taurine and glycerophosphorylcholine) balances in neuron-like cells and of metabolic waste products (namely lactate, ethanol and acetate), glycine and glutamine in their growth media. These results may contribute to gain more knowledge about the IFN-α2 induced effect on the brain and support the interpretation of magnetic resonance spectroscopy studies performed in humans. Keywords IFN-α SH-SY5Y cells Metabolomics HR-MAS NMR Osmolytes Neurotoxicity