Magnetic Capture of a Molecular Biomarker from Synovial Fluid in a Rat Model of Knee Osteoarthritis

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• 作者：Elena G. Yarmola ; Yash Shah ; David P. Arnold…
• 关键词：Magnetic nanoparticles ; Biomarkers ; Synovial fluid ; Osteoarthritis ; Rodent model
• 刊名：Annals of Biomedical Engineering
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：44
• 期：4
• 页码：1159-1169
• 全文大小：1,943 KB
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• 作者单位：Elena G. Yarmola (1) (6)
  Yash Shah (1)
  David P. Arnold (3) (4) (5)
  Jon Dobson (1) (2) (4) (5)
  Kyle D. Allen (1) (4) (5) (7)
  
  1. J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, 1275 Center Dr, Biomedical Sciences Building, JG56, Gainesville, FL, 32610, USA
  6. Department of Medicine, University of Florida, 1600 SW Archer Rd, Gainesville, FL, 32610, USA
  3. Department of Electrical and Computer Engineering, University of Florida, 216 Larsen Hall, Gainesville, FL, 32611, USA
  4. Institute for Cell Engineering and Regenerative Medicine, University of Florida, 300 Weil Hall, Gainesville, FL, 32611, USA
  5. Nanoscience Institute for Medical and Engineering Technology (NIMET), University of Florida, 1041 Center Dr, Gainesville, FL, 32611, USA
  2. Department of Materials Science and Engineering, University of Florida, 100 Rhines Hall, Gainesville, FL, 32611, USA
  7. Department of Biomedical Engineering, University of Florida, PO Box 116131, Gainesville, FL, 32611, USA
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Biomedicine
  Biomedical Engineering
  Biophysics and Biomedical Physics
  Mechanics
  Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-9686

文摘

Biomarker development for osteoarthritis (OA) often begins in rodent models, but can be limited by an inability to aspirate synovial fluid from a rodent stifle (similar to the human knee). To address this limitation, we have developed a magnetic nanoparticle-based technology to collect biomarkers from a rodent stifle, termed magnetic capture. Using a common OA biomarker—the c-terminus telopeptide of type II collagen (CTXII)—magnetic capture was optimized in vitro using bovine synovial fluid and then tested in a rat model of knee OA. Anti-CTXII antibodies were conjugated to the surface of superparamagnetic iron oxide-containing polymeric particles. Using these anti-CTXII particles, magnetic capture was able to estimate the level of CTXII in 25 μL aliquots of bovine synovial fluid; and under controlled conditions, this estimate was unaffected by synovial fluid viscosity. Following in vitro testing, anti-CTXII particles were tested in a rat monoiodoacetate model of knee OA. CTXII could be magnetically captured from a rodent stifle without the need to aspirate fluid and showed tenfold changes in CTXII levels from OA-affected joints relative to contralateral control joints. Combined, these data demonstrate the ability and sensitivity of magnetic capture for post-mortem analysis of OA biomarkers in the rat.