Vesicular transport system in myotubes: ultrastructural study and signposting with vesicle-associated membrane proteins

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• 作者：Yuki Tajika (1)
  Maiko Takahashi (1)
  Astrid Feinisa Khairani (1)
  Hitoshi Ueno (1)
  Tohru Murakami (1)
  Hiroshi Yorifuji (1)
  
• 关键词：Skeletal muscle ; Myotube ; SNARE ; VAMP ; T ; tubule
• 刊名：Histochemistry and Cell Biology
• 出版年：2014
• 出版时间：April 2014
• 年：2014
• 卷：141
• 期：4
• 页码：441-454
• 全文大小：1,515 KB
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• 作者单位：Yuki Tajika (1)
  Maiko Takahashi (1)
  Astrid Feinisa Khairani (1)
  Hitoshi Ueno (1)
  Tohru Murakami (1)
  Hiroshi Yorifuji (1)
  
  1. Department of Anatomy, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma, 371-8511, Japan
  
• ISSN：1432-119X

文摘

Myofibers have characteristic membrane compartments in their cytoplasm and sarcolemma, such as the sarcoplasmic reticulum, T-tubules, neuromuscular junction, and myotendinous junction. Little is known about the vesicular transport that is believed to mediate the development of these membrane compartments. We determined the locations of organelles in differentiating myotubes. Electron microscopic observation of a whole myotube revealed the arrangement of Golgi apparatus, rough endoplasmic reticulum, autolysosomes, mitochondria, and smooth endoplasmic reticulum from the perinuclear region toward the end of myotubes and the existence of a large number of vesicles near the ends of myotubes. Vesicles in myotubes were further characterized using immunofluorescence microscopy to analyze expression and localization of vesicle-associated membrane proteins (VAMPs). VAMPs are a family of seven proteins that regulate post-Golgi vesicular transport via the fusion of vesicles to the target membranes. Myotubes express five VAMPs in total. Vesicles with VAMP2, VAMP3, or VAMP5 were found near the ends of the myotubes. Some of these vesicles are also positive for caveolin-3, suggesting their participation in the development of T-tubules. Our morphological analyses revealed the characteristic arrangement of organelles in myotubes and the existence of transport vesicles near the ends of the myotubes.