Cellular reactions to biodegradable magnesium alloys on human growth plate chondrocytes and osteoblasts
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- 作者：Karin Pichler (1) (5)
Tanja Kraus (2)
Elisabeth Martinelli (1)
Patrick Sadoghi (1)
Giuseppe Musumeci (3)
Peter J. Uggowitzer (4)
Annelie M. Weinberg (1) - 关键词：Biodegradable magnesium ; Orthopaedics ; Immature skeleton ; Growth ; Biocompatibility
- 刊名：International Orthopaedics
- 出版年：2014
- 出版时间：April 2014
- 年：2014
- 卷：38
- 期：4
- 页码：881-889
- 全文大小：990 KB
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Tanja Kraus (2)
Elisabeth Martinelli (1)
Patrick Sadoghi (1)
Giuseppe Musumeci (3)
Peter J. Uggowitzer (4)
Annelie M. Weinberg (1)
1. Department of Orthopaedic Surgery, Medical University of Graz, Auenbruggerplatz 5, 8036, Graz, Austria
5. Department of Orthopaedic Surgery, Medical University of Graz, Auenbruggerplatz 36, 8036, Graz, Austria
2. Department of Paediatric Orthopaedic Surgery, Medical University of Graz, Auenbruggerplatz 34, 8036, Graz, Austria
3. Department of Bio-Medical Sciences, University of Catania, Via S.Sofia 87, 95100, Catania, Italy
4. Department of Materials, ETH Zurich, Wolfgang-Pauli-Str. 10, 8093, Zurich, Switzerland - ISSN：1432-5195
文摘
Purpose In recent decades operative fracture treatment using elastic stable intramedullary nails (ESINs) has mainly taken precedence over conservative alternatives in children. The development of biodegradable materials that could be used for ESINs would be a further step towards treatment improvement. Due to its mechanical and elastic properties, magnesium seems to be an ideal material for biodegradable implant application. The aim of this study was therefore to investigate the cellular reaction to biodegradable magnesium implants in vitro. Methods Primary human growth plate chondrocytes and MG63 osteoblasts were used for this study. Viability and metabolic activity in response to the eluate of a rapidly and a slower degrading magnesium alloy were investigated. Furthermore, changes in gene expression were assessed and live cell imaging was performed. Results A superior performance of the slower degrading WZ21 alloy’s eluate was detected regarding cell viability and metabolic activity, cell proliferation and morphology. However, the ZX50 alloy’s eluate induced a favourable up-regulation of osteogenic markers in MG63 osteoblasts. Conclusions This study showed that magnesium alloys for use in biodegradable implant application are well tolerated in both osteoblasts and growth plate chondrocytes respectively.