Influence of nitrogen deficiency on biochemical composition of the green alga Botryococcus

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• 作者：Natalia O. Zhila (1)
  Galina S. Kalacheva (1)
  Tatiana G. Volova (1)
  
• 关键词：Botryococcus ; fatty acids ; lipids ; nitrogen deficiency
• 刊名：Journal of Applied Phycology
• 出版年：2005
• 出版时间：June 2005
• 年：2005
• 卷：17
• 期：4
• 页码：309-315
• 全文大小：427KB
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• 作者单位：Natalia O. Zhila (1)
  Galina S. Kalacheva (1)
  Tatiana G. Volova (1)
  
  1. Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Akademgorodok, 660036, Krasnoyarsk, Russia
  

文摘

A study has been carried out to investigate the influence of nitrogen deficiency on intracellular lipid composition, including total fatty acid composition of lipids, polar lipids, and triacylglycerols, of the alga Botryococcus braunii Kütz IPPAS H-252 in batch culture. Under nitrogen limitation, the alga accumulates lipids as triacylglycerols and the total fatty acid (FA) composition changes: trienoic acids decrease (from 52.8-7.2 to 19.5-4.7% of the total FAs) and the oleic acid increases (from 1.1-.2 to 17.1-4.4%) as does the saturated acids (from 23.7-6 to 32.9-6.1%). A similar rearrangement in the FA spectrum occurs at later times in the control culture, but it is less pronounced. Under nitrogen limitation, considerable changes in the polar lipid FAs are registered at day 13: saturated acids increase (from 28.6-5.5 to 76.8%) and all polyenoic acids markedly decrease (from 56.9-4.1 to 6.8%). Changes in the triacylglycerol fatty acid spectrum are seen on day 7: the oleic acid increases (from 14.7 to 34.2%) and remains at a high level till the end of the culture. In the control, triacylglycerols with large contents of oleic acid are detected at day 13, the total lipids and triacylglycerols still remaining unchanged.