Age-Related Changes in DNA Methylation Associated with Shifting Th1/Th2 Balance
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- 作者：Yu Xia ; Jun Yang ; Guobin Wang ; Chengrong Li ; Qiu Li
- 关键词：KEY WORDSepigenetics ; methylation ; T helper cell
- 刊名：Inflammation
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：39
- 期：6
- 页码：1892-1903
- 全文大小：2,017 KB
- 刊物类别：Medicine
- 刊物主题：Medicine & Public Health
Rheumatology
Internal Medicine
Pharmacology and Toxicology
Pathology - 出版者：Springer Netherlands
- ISSN：1573-2576
- 卷排序：39
文摘
This study was conducted in order to explore age-related changes in the production of Th1 and Th2 cytokines and determine the corresponding status of DNA methylation. The plasma IL-4 and IFN-γ levels and expression of Th-related cytokines and transcription factors in CD4+ splenocytes were observed in mice at different weeks of age. The DNA methylation levels of IL-4 and IFN-γ promoters and the related regulatory regions in CD4+ splenocytes of mice at different weeks of age were analyzed. The DNA methyltransferase (DNMT) levels in CD4+ splenocytes of mice were analyzed. Changes in plasma IL-4 and IFN-γ levels after 5-AZA injection were evaluated. Plasma IL-4 and IL-4 expression in CD4+ splenocytes declined with increasing age, while the IFN-γ expression levels increased. Th-related transcription factors showed no differences in mice at different weeks of age. The DNMT1 and DNMT3b mRNA expression did not show significant changes in CD4+ splenocytes, whereas the DNMT3a mRNA expression increased with age. DNA methylation in the IL-4 promoter was increased, while DNA methylation in the IFN-γ promoter was decreased. The methylation of RSH7, CNS-1, and HSV increased significantly with age. Age-related changes in DNA methylation may be associated with the shift in Th1/Th2 balance.