Effects of native and oxidized apolipoprotein A-I on lipid bilayer microviscosity of erythrocyte plasma membrane

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• 作者：P. V. Mokrushnikov ; A. N. Dudarev…
• 关键词：erythrocyte ; plasma membrane ; apolipoprotein A ; I ; oxidative modification of proteins ; tetrahydrocortisol ; fluorescence analysis ; membrane microviscosity
• 刊名：Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：11
• 期：1
• 页码：48-53
• 全文大小：
• 刊物主题：Cell Biology;
• 出版者：Pleiades Publishing
• ISSN：1990-7494
• 卷排序：11

文摘

Using a pyrene as a fluorescent probe, we investigated the influence of native and oxidized apolipoprotein A-I (apo A-I) and their complexes with tetrahydrocortisol (THC) on the microviscosity of the erythrocyte plasma membrane. The addition of THC to isolated membranes led to a 17% increase in the membrane microviscosity. In contrast, native apo A-I reduced the microviscosity (i.e., increased the fluidity) of the membranes by 15%. A more pronounced increase (by 25%) in the membrane fluidity was found in the presence of the complex of apo A-I with THC. Unlike native apo A-I, oxidized apo A-I and its complex with THC did not change the membrane viscosity. In view of the fact that apo A-I plays an important role in the binding of membrane cholesterol we suggest that the observed increase in the membrane fluidity under the influence of the native apo A-I is associated with the cholesterol efflux from plasma membrane. Oxidative modification of apo A-I likely disturbs the mechanisms of the cholesterol efflux and prevents the decrease in the membrane microviscosity.