Simple protein precipitation-based analysis of Δ9-tetrahydrocannabinol and its metabolites in human serum by liquid chromatography–tandem mass spectrometry
详细信息 查看全文
- 作者：Marek Dziadosz ; Michael Klintschar ; Jörg Teske
- 关键词：Δ9 ; Tetrahydrocannabinol ; THC ; Cannabis ; LC–MS/MS ; Protein precipitation ; Fast ; simple and cost effective
- 刊名：Forensic Toxicology
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：35
- 期：1
- 页码：190-194
- 全文大小：
- 刊物主题：Forensic Medicine; Pharmacology/Toxicology; Medicinal Chemistry; Medicine/Public Health, general; Medical Law;
- 出版者：Springer Japan
- ISSN：1860-8973
- 卷排序：35
文摘
A liquid chromatography–tandem mass spectrometry (LC–MS/MS) quantification method based on a direct protein precipitation with the mobile phase was developed, validated and applied for the analysis of Δ9-tetrahydrocannabinol (THC), 11-hydroxy-Δ9-tetrahydrocannabinol (11-OH-THC) and 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THC-COOH) in human serum for forensic purposes. Chromatographic separation was performed with a Luna 5 µm C18 (2) 100 A, 150 × 2 mm analytical column and the elution with a programmed flow of a mobile phase consisting of A (H2O/methanol, 95:5, v/v) and B (H2O/methanol, 3:97, v/v), both with 10 mM ammonium acetate and 0.1 % acetic acid. The results of the validation experiments demonstrated that the strategy presented can be applied successfully for a fast, simple and cost effective LC–MS/MS analysis of THC and its metabolites. A potential conversion of THC-COOH glucuronide to THC-COOH during sample preparation/analysis could be excluded.