Construction of a molecular functional map of rapeseed (Brassica napus L.) using differentially expressed genes between hybrid and its parents
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- 作者：Yuanyuan Li ; Chaozhi Ma ; Tingdong Fu ; Guangsheng Yang ; Jingxing Tu ; Qingfang Chen ; Tonghua Wang ; Xingguo Zhang and Chunyan Li
- 关键词：B. napus ; Comparative mapping ; Differentially expressed genes ; Molecular functional map ; SSCP
- 刊名：Euphytica
- 出版年：2006
- 出版时间：November, 2006
- 年：2006
- 卷：152
- 期：1
- 页码：25-39
- 全文大小：406 KB
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Plant Physiology
Plant Sciences
Ecology - 出版者：Springer Netherlands
- ISSN：1573-5060
文摘
Brassica napus L. is an important oilseed and fodder crop with significant heterosis for seed yield and other agronomic traits, but very little is known about the molecular basis of heterosis. As an initial step towards understanding the molecular events associated with this phenomenon, a molecular functional map of rapeseed was constructed using differentially expressed genes in hybrid identified by microarrays. Single-strand conformational polymorphism (SSCP) analysis was applied for genetic mapping in an F 2 population of 184 individuals resulting from crossing ‘`SI-1300× Eagle’'. A total of 162 markers including 154 loci corresponding to 98 differentially expressed genes assigned to 17 functional categories and 8 SSR markers were grouped into 21 linkage groups (LGs), covering a total map distance of 2267.3 cM. Subsequently, this map was aligned with Arabidopsis thaliana in silico. Comparative mapping shows that genes localized on each Arabidopsis chromosome have orthologs dispreading in different B. napus LGs. Similarly, a majority of LGs were made of homologous genes from different Arabidopsis chromosomes. In addition, a total of 25 syntenic regions were identified in B. napus, in most of which the gene order was not consistent between the two species, and each of the conserved regions in the A. thaliana genome was homologous to 1--5 distinct regions in the B. napus genome. These results indicate that it is not easy to exploit A. thaliana information for B. napus based on synteny.