Canine urothelial carcinoma: genomically aberrant and comparatively relevant

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• 作者：S. G. Shapiro ; S. Raghunath ; C. Williams ; A. A. Motsinger-Reif…
• 关键词：Canine ; Urothelial carcinoma ; Transitional cell carcinoma ; Cytogenetics ; Chromosome aberration ; Array comparative genomic hybridization ; Comparative oncology
• 刊名：Chromosome Research
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：23
• 期：2
• 页码：311-331
• 全文大小：5,692 KB
• 参考文献：American Cancer Society (2014) Cancer facts and figures 2014. Society AC, Atlanta<br>American Joint Committee on Cancer (2002) Urinary bladder. In: Greene F, Page D, Fleming I et al (eds) Cancer staging manual. Springer-Verlag, New York, pp 335-38View Article <br>Anderson W, Dunham B, King J et al (1989) Presumptive subcutaneous transplation of a urinary bladder transitional cell carcinoma of the urinary bladder in a dog. Cornell Vet 79:263-66PubMed <br>Angstadt AY, Motsinger-Reif A, Thomas R et al (2011) Characterization of canine osteosarcoma by array comparative genomic hybridization and RT-qPCR: signatures of genomic imbalance in canine osteosarcoma parallel the human counterpart. Genes Chromosom Cancer 50(11):859-74View Article PubMed <br>Angstadt AY, Thayanithy V, Subramanian S, Modiano JF, Breen M (2012) A genome-wide approach to comparative oncology: high-resolution oligonucleotide aCGH of canine and human osteosarcoma pinpoints shared microaberrations. Cancer Genet 205(11):572-87View Article PubMed <br>Bansal N, Gupta A, Sankhwar SN, Mahdi AA (2014) Low- and high-grade bladder cancer appraisal via serum-based proteomics approach. Clin Chim Acta 436:97-03View Article PubMed <br>Bhatlekar S, Fields JZ, Boman BM (2014) HOX genes and their role in the development of human cancers. J Mol Med (1432-440 (Electronic))<br>Cai H, Kumar N, Bagheri H, von Mering C, Robinson M, Baudis M (2014) Chromothripsis-like patterns are recurring but heterogenously distributed features in a survery of 22,347 cancer genome screens. BMC Genomics 15:82View Article PubMed Central PubMed <br>Calin GA, Dumitru CS et al (2004) Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers. Proc Natl Acad Sci 101(9):2999-004View Article PubMed Central PubMed <br>Castillo-Martin M, Domingo-Domenech J, Karni-Schmidt O, Matos T, Cordon-Cardo C (2010) Molecular pathways of urothelial development and bladder tumorigenesis. Urol Oncol 28(4):401-08View Article PubMed <br>Chekaluk Y, Wu CL, Rosenberg J et al (2013) Identification of nine genomic regions of amplification in urothelial carcinoma, correlation with stage, and potential prognostic and therapeutic value. PLoS One 8(4):e60927View Article PubMed Central PubMed <br>Chen R, Feng C, Xu Y (2011) Cyclin-dependent kinase-associated protein Cks2 is associated with bladder cancer progression. J Int Med Res 39(2):533-40View Article PubMed <br>Development Team R (2010) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna<br>Dhawan D, Ramos-Vara JA, Stewart JC, Zheng R, Knapp DW (2009) Canine invasive transitional cell carcinoma cell lines: in vitro tools to complement a relevant animal model of invasive urinary bladder cancer. Urol Oncol 27(3):284-92View Article PubMed <br>Dobson JM (2013) Breed-predispositions to cancer in pedigree dogs. ISRN Veterinary Science 2013<br>Ebbing J, Mathia S, Seibert F et al (2013) Urinary calprotectin: a new diagnostic marker in urothelial carcinoma of the bladder. World J Urol 1-<br>Eliseeva IA, Lyabin DN, Ovchinnikov LP (2013) Poly(A)-binding proteins: structure, domain organization, and activity regulation. Biochemistry Moscow 78(13):1377-391View Article PubMed <br>Fadl-Elmula I (2005) Chromosomal changes in uroepithelial carcinomas. Cell Chromosom 4:1View Article <br>Goebell PJ, Knowles MA (2010) Bladder cancer or bladder cancers? Genetically distinct malignant conditions of the urothelium. Urol Oncol 28(4):409-28View Article PubMed <br>Hall M, Frank E, Holmes G, Pfahringer B, Reutemann P, Witten IH (2009) The WEKA Data Mining Software: an update. SIGKDD Explorations 11(1)<br>Hedan B, Thomas R, Motsinger-Reif A et al (2011) Molecular cytogenetic characterization of canine histiocytic sarcoma: a spontaneous model for human histiocytic cancer identifies deletion of tumor suppressor genes and highlights influence of genetic background on tumor behavior. BMC Cancer (in revision)<br>Heidenblad M, Lindgren D, Jonson T et al (2008) Tiling resolution array CGH and high density expression profiling of urothelial carcinomas delineate genomic amplicons and candidate target genes specific for advanced tumors. BMC Med Genomics 1:3View Article PubMed Central PubMed <br>Hosseini SA, Horton S, Saldivar JC et al (2013) Common chromosome fragile sites in human and murine epithelial cells and FHIT/FRA3B loss-induced global genome instability. Genes Chromosom Cancer 52(11):1017-029View Article PubMed Central PubMed <br>Karolchik D, Hinrichs AS, Furey TS et al (2004) The UCSC Table Browser data retrieval tool. Nucleic Acids Res 32(suppl 1):D493–D496View Article PubMed Central PubMed <br>Kim WJ, Kim SK, Jeong P et al (2011) A four-gene signature predicts disease progression in muscle invasive bladder cancer. Mol Med 17(5-):478-85PubMed Central PubMed <br>Kim WT, Kim J, Yan C et al (2014) S100A9 and EGFR gene signatures predict disease progression in muscle invasive bladde
• 作者单位：S. G. Shapiro (1) <br> S. Raghunath (1) (2) <br> C. Williams (1) <br> A. A. Motsinger-Reif (3) (8) <br> J. M. Cullen (4) (8) <br> T. Liu (5) <br> D. Albertson (5) <br> M. Ruvolo (6) <br> A. Bergstrom Lucas (6) <br> J. Jin (6) <br> D. W. Knapp (7) <br> J. D. Schiffman (2) <br> M. Breen (1) (10) (8) (9) <br><br>1. Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC, 27607, USA <br> 2. Department of Pediatrics and Huntsman Cancer Institute, University of Utah, 2000 Circle of Hope, Salt Lake City, UT, 84112, USA <br> 3. Department of Statistics, College of Sciences, North Carolina State University, Raleigh, NC, USA <br> 8. Center for Comparative Medicine and Translational Research, North Carolina State University, Raleigh, NC, USA <br> 4. Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA <br> 5. Anatomic Pathology Division Department of Pathology, University of Utah, 1950 Circle of Hope, RM N3105, Salt Lake City, UT, 84112, USA <br> 6. Agilent Technologies, 5301 Stevens Creek Blvd., Santa Clara, CA, 95051, USA <br> 7. Department of Veterinary Clinical Sciences, Purdue University, School of Veterinary Medicine, West Lafayette, IN, USA <br> 10. Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC, USA <br> 9. Center for Human Health and the Environment, North Carolina State University, Raleigh, NC, USA <br>
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences<br>Cell Biology<br>Human Genetics<br>Animal Genetics and Genomics<br>Plant Genetics and Genomics<br>
• 出版者：Springer Netherlands
• ISSN：1573-6849

文摘

Urothelial carcinoma (UC), also referred to as transitional cell carcinoma (TCC), is the most common bladder malignancy in both human and canine populations. In human UC, numerous studies have demonstrated the prevalence of chromosomal imbalances. Although the histopathology of the disease is similar in both species, studies evaluating the genomic profile of canine UC are lacking, limiting the discovery of key comparative molecular markers associated with driving UC pathogenesis. In the present study, we evaluated 31 primary canine UC biopsies by oligonucleotide array comparative genomic hybridization (oaCGH). Results highlighted the presence of three highly recurrent numerical aberrations: gain of dog chromosome (CFA) 13 and 36 and loss of CFA 19. Regional gains of CFA 13 and 36 were present in 97 %?and 84?% of cases, respectively, and losses on CFA 19 were present in 77?% of cases. Fluorescence in situ hybridization (FISH), using targeted bacterial artificial chromosome (BAC) clones and custom?Agilent SureFISH probes, was performed?to detect and quantify these regions in paraffin-embedded biopsy sections and urine-derived urothelial cells. The data indicate that these three aberrations are potentially diagnostic of UC. Comparison of our canine oaCGH data with that of 285 human cases identified a series of shared copy number aberrations. Using an informatics approach to interrogate the frequency of copy number aberrations across both species, we identified those that had the highest joint probability of association with UC. The most significant joint region contained the gene PABPC1, which should be considered further for its role in UC progression. In addition, cross-species filtering of genome-wide copy number data highlighted several genes as high-profile candidates for further analysis, including CDKN2A, S100A8/9, and LRP1B. We propose that these common aberrations are indicative of an evolutionarily conserved mechanism of pathogenesis and harbor genes key to urothelial neoplasia, warranting investigation for diagnostic, prognostic, and therapeutic applications.