Poly(2-substituted-2-oxazoline) surfaces for dermal fibroblasts adhesion and detachment

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• 作者：Andrzej Dworak (1)
  Alicja Utrata-Weso?ek (1)
  Natalia Oleszko (1)
  Wojciech Wa?ach (1)
  Barbara Trzebicka (1)
  Jacek Anio? (2)
  Aleksander L. Sieroń (2)
  Agnieszka Klama-Bary?a (3)
  Marek Kawecki (3)
  
• 刊名：Journal of Materials Science Materials in Medicine
• 出版年：2014
• 出版时间：April 2014
• 年：2014
• 卷：25
• 期：4
• 页码：1149-1163
• 全文大小：2,886 KB
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• 作者单位：Andrzej Dworak (1)
  Alicja Utrata-Weso?ek (1)
  Natalia Oleszko (1)
  Wojciech Wa?ach (1)
  Barbara Trzebicka (1)
  Jacek Anio? (2)
  Aleksander L. Sieroń (2)
  Agnieszka Klama-Bary?a (3)
  Marek Kawecki (3)
  
  1. Center of Polymer and Carbon Materials, Polish Academy of Sciences, M. Curie-Sklodowskiej 34, 41-819, Zabrze, Poland
  2. Department of General, Molecular Biology and Genetics, Medical University of Silesia, Medykow 18, 40-752, Katowice, Poland
  3. Center for Burn Treatment, Jana Pawla II, 41-100, Siemianowice Slaskie, Poland
  
• ISSN：1573-4838

文摘

The thermoresponsive surfaces of brush structure (linear polymer chains tethered on the surface) based on poly(2-isopropyl-2-oxazoline)s and copolymers of 2-ethyl-2-oxazoline and 2-nonyl-2-oxazoline were obtained using the grafting-to method. The living oxazoline (co)polymers have been synthesized by cationic ring-opening polymerization and subsequently terminated by the reactive amine groups present on the surface. The changes in the surface morphology, philicity and thickness occurring during surface modification were monitored via atomic force microscopy, contact angle and ellipsometry. The thickness of the (co)poly(2-substituted-2-oxazoline) layers ranged from 4 to 11?nm depending on the molar mass of immobilized polymer and reversibly varied with the temperature changes. This confirmed thermoresponsive properties of obtained surfaces. The obtained polymer surfaces were used as a support for dermal fibroblast culture and detachment. The fibroblasts-adhesion and proliferation on the polymer surfaces were observed when the culture temperature was above the cloud point temperature of the immobilized polymer. Lowering the temperature resulted in the detachment of the dermal fibroblast sheets from the polymer layers, which makes these surfaces suitable for the treatment of wounds and in skin tissue engineering.