Development of microsatellite markers in cultivated and wild species of sections Cepa and Phyllodolon in Allium

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• 作者：Naoyuki Araki ; Shin-Ichi Masuzaki ; Hikaru Tsukazaki ; Shigenori Yaguchi ; Tadayuki Wako ; Yosuke Tashiro ; Naoki Yamauchi and Masayoshi Shigyo
• 关键词：Allium ; Microsatellite markers ; DNA polymorphism
• 刊名：Euphytica
• 出版年：2010
• 出版时间：June, 2010
• 年：2010
• 卷：173
• 期：3
• 页码：321-328
• 全文大小：356.4 KB

文摘

The potential of microsatellite markers for use in genetic studies has been evaluated in Allium cultivated species (Allium cepa, A. fistulosum) and its allied species (A. altaicum, A. galanthum, A. roylei, A. vavilovii). A total of 77 polymerase chain reaction (PCR) primer pairs were employed, 76 of which amplified a single product or several products in either of the species. The 29 AMS primer pairs derived from A. cepa and 46 microsatellites primer pairs from A. fistulosum revealed a lot of polymorphic amplicons between seven Allium species. Some of the microsatellite markers were effective not only for identifying an intraspecific F₁ hybrid between shallot and bulb onion but also for applying to segregation analyses in its F₂ population. All of the microsatellite markers can be used for interspecific taxonomic analyses among two cultivated and four wild species of sections Cepa and Phyllodolon in Allium. Generally, our data support the results obtained from recently performed analyses using molecular and morphological markers. However, the phylogeny of A. roylei, a threatened species with several favorable genes, was still ambiguous due to its different positions in each dendrogram generated from the two primer sets originated from A. cepa and A. fistulosum.