Bauhinia bauhinioides cruzipain inhibitor reduces endothelial proliferation and induces an increase of the intracellular Ca2+ concentration
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- 作者：Mehmet Bilgin (1)
Christiane Neuhof (1)
Oliver Doerr (1)
Utz Benscheid (1)
Sheila S. Andrade (3)
Astrid Most (1)
Yaser Abdallah (2)
Mariana Parahuleva (1)
Dursun Guenduez (1)
Maria L. Oliva (3)
Ali Erdogan (1) - 关键词：Bauhinia bauhinioides cruzipain inhibitor ; Endothelial cells ; Calcium ; Hyperpolarization ; Proliferation
- 刊名：Journal of Physiology and Biochemistry
- 出版年：2010
- 出版时间：December 2010
- 年：2010
- 卷：66
- 期：4
- 页码：283-290
- 全文大小：207KB
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Christiane Neuhof (1)
Oliver Doerr (1)
Utz Benscheid (1)
Sheila S. Andrade (3)
Astrid Most (1)
Yaser Abdallah (2)
Mariana Parahuleva (1)
Dursun Guenduez (1)
Maria L. Oliva (3)
Ali Erdogan (1)
1. Department of Cardiology and Angiology, Justus-Liebig-University of Giessen, Klinikstrasse 36, 35392, Giessen, Germany
3. Department of Biochemistry, Universidade Federal de S?o Paulo, S?o Paulo, Brazil
2. Institute of Physiology, Justus-Liebig-University of Giessen, Giessen, Germany - ISSN：1877-8755
文摘
Proteinase inhibitors, isolated from different types of Bauhinia, have an effect on apoptosis, angiogenesis and inflammation. The Bauhinia bauhinioides cruzipain inhibitor (BbCI) is a Kunitz-type inhibitor and inactivates the cysteine proteinases cruzipain and cruzain from Trypanosoma cruzi. Cruzipain and tissue kallikrein have similar biochemical properties, e.g. the proteolytic cleavage of the kininogen precursor of lys-bradykinin. Tissue kallikrein stimulation in endothelial cells causes migration and capillary tube formation. The aim of this study was to examine whether the antiproliferative effect of BbCI is dependent on changes of the intracellular calcium concentration and membrane hyperpolarization. Endothelial cells were isolated from human umbilical cord veins (HUVEC). For proliferation experiments, HUVEC were incubated with BbCI (10-00?μmol/L) for 48?h. The proliferation was detected by cell counting with a Neubauer chamber. The effect of BbCI (10-00?μM) on the membrane potential was measured with the fluorescence dye DiBAC4(3) and the effect on [Ca+2] i with the fluorescence probe Fluo-3 AM. The change of the fluorescence intensity was determined with a GENios plate reader (Tecan). The experiments showed that BbCI (10-00?μmol/L) reduces the endothelial cell proliferation significantly in a concentration-dependent manner with a maximum effect at 100?μmol/L (35.1?±-.8% as compared to control (p?≤-.05; n--5)). As compared to the control, the addition of BbCI (100?μmol/L) caused a significant increase of systolic Ca2+ of 28.4?±-.0% after 30?min incubation. HUVEC treatment with BbCI (100?μmol/L) showed a weak but significant decrease of the membrane potential of 9.5?±-.9% as compared to control (p?≤-.05; n--0). BbCI influenced significantly the endothelial proliferation, the intracellular Ca2+ concentration and the membrane potential.