Arginase activity, urea, and hydroxyproline concentration are reduced in keratoconus keratocytes
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- 作者：Tanja Stachon ; Krasimir Kolev ; Zsuzsa Flaskó…
- 关键词：Urea ; Keratoconus ; Keratocytes ; Arginase ; Hydroxyproline concentration ; Viability ; Proliferation
- 刊名：Graefe's Archive for Clinical and Experimental Ophthalmology
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：255
- 期：1
- 页码：91-97
- 全文大小：
- 刊物类别：Medicine
- 刊物主题：Ophthalmology;
- 出版者：Springer Berlin Heidelberg
- ISSN：1435-702X
- 卷排序：255
文摘
PurposeKeratoconus (KC) is a disease characterized by thinning and deformation of the cornea, but its etiology remains unknown. Seventy percent of the corneal stroma consists of collagen, which is composed of three intertwined polypeptide chains with glycine-hydroxyproline-proline repeats along their sequence. Arginase is a cytoplasmatic enzyme and catalyzes the conversion of arginine to urea and ornithine, which serves as a precursor for the endogenous synthesis of proline and hydroxyproline. The purpose of this study was to analyze arginase activity, as well as collagen and urea formation in normal and KC-keratocytes and to determine the impact of urea on keratocyte viability and proliferation in vitro.