Vaspin inhibits cytokine-induced nuclear factor-kappa B activation and adhesion molecule expression via AMP-activated protein kinase activation in vascular endothelial cells

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• 作者：Chang Hee Jung (1)
  Min Jung Lee (1)
  Yu Mi Kang (1)
  Yoo La Lee (2)
  Hae Kyeong Yoon (2)
  Sang-Wook Kang (3)
  Woo Je Lee (1)
  Joong-Yeol Park (1)
  
• 关键词：Vaspin ; Endothelial cells ; AMPK ; NF ; κB ; Adhesion molecules
• 刊名：Cardiovascular Diabetology
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：13
• 期：1
• 全文大小：687 KB
• 作者单位：Chang Hee Jung (1)
  Min Jung Lee (1)
  Yu Mi Kang (1)
  Yoo La Lee (2)
  Hae Kyeong Yoon (2)
  Sang-Wook Kang (3)
  Woo Je Lee (1)
  Joong-Yeol Park (1)
  
  1. Department of Internal Medicine, University of Ulsan College of Medicine, Poongnap-dong, Songpa-gu, Seoul, 138-736, Korea
  2. Asan Institute of Life Sciences, University of Ulsan College of Medicine, Seoul, Korea
  3. Department of Biomedical Sciences, University of Ulsan College of Medicine, Seoul, Korea
  
• ISSN：1475-2840

文摘

Background Vaspin is an adipocytokine that was recently identified in the visceral adipose tissue of diabetic rats and has anti-diabetic and anti-atherogenic effects. We hypothesized that vaspin prevents inflammatory cytokine-induced nuclear factor-kappa B (NF-κB) activation by activating AMP-activated protein kinase (AMPK) in vascular endothelial cells. Methods We examined the effects of vaspin on NF-κB activation and the expression of the NF-κB-mediated genes intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), E-selectin, and monocyte chemoattractant protein-1 (MCP-1). Human aortic endothelial cells (HAECS) were used. Tumor necrosis factor alpha (TNFα) was used as a representative proinflammatory cytokine. Results Treatment with vaspin significantly increased the phosphorylation of AMPK and acetyl-CoA carboxylase, the down-stream target of AMPK. Furthermore, treatment with vaspin significantly decreased TNFα-induced activation of NF-κB, as well as the expression of the adhesion molecules ICAM-1, VCAM-1, E-selectin, and MCP-1. These effects were abolished following transfection of AMPKα1-specific small interfering RNA. In an adhesion assay using THP-1 cells, vaspin reduced TNFα-induced adhesion of monocytes to HAECS in an AMPK-dependent manner. Conclusions Vaspin might attenuate the cytokine-induced expression of adhesion molecule genes by inhibiting NF-κB following AMPK activation.